菜籽油中芥酸含量对小鼠食用安全性的影响.PDF

※营养卫生 食品科学 2010, Vol. 31, No. 19 321 雷 红，蔡亮亮，操丽丽，姜绍通 (合肥工业大学生物与食品工程学院，安徽 合肥 230009) 采用气相色谱法分析双低冷榨菜籽油和普通菜籽油的芥酸含量；运用掺食法喂饲小鼠含两种菜籽油的饲料 (含油量2%) ，连续饲养5 周，观察小鼠、体质量、摄食量、总胆固醇(TC) 、甘油三酯(TG) 、高密度脂蛋白(HDL-C) 、 脏器( 心、肝、脾、肺、双肾、睾丸、卵巢) 质量的变化；研究菜籽油中芥酸含量对小鼠食用安全性的影响。 结果表明，双低冷榨菜籽油芥酸含量为4.744% ，普通菜籽油芥酸含量为 32.595% ，双低冷榨菜籽油芥酸含量明显 低于普通菜籽油。喂饲双低冷榨菜籽油饲料组小鼠血清 TC 、TG 水平低于普通菜籽油饲料组；血清 HDL-C 水平略 高于普通菜籽油饲料组；与普通菜籽油饲料组相比，喂饲双低冷榨菜籽油饲料对小鼠血脂水平有良好影响。与正 常对照组和双低冷榨菜籽油组相比，普通菜籽油组小鼠心脏质量和肝脏质量明显升高，可能与芥酸使脂肪沉积于心 脏和肝脏有关。喂饲两种菜籽油对小鼠体质量、摄食、其他脏器质量均无明显影响。 菜籽油；芥酸含量；血脂；脏器质量；食用安全性 Effect of Erucic Acid Content in Rapeseed Oil on Food Intake Safety in Mice LEI Hong ，CAI Liang-liang ，CAO Li-li ，JIANG Shao-tong (College of Biological and Food Engineering , Hefei University of Technology, Hefei 230009, China) Abstract ：The erucic acid contents of cold-pressing double-low rapeseed oil and common rapeseed oil were analyzed by gas chromatography (GC). Sixty Kunming mice (half male and half female) were divided into 3 groups of 20 mice each (also half male and half female): control (fed normal diet), cold-pressing double-low rapeseed oil-treated (fed diet containing cold-pressing double-low rapeseed oil with 2% oil content) and common rapeseed oil-treated (fed common rapeseed oil with 2% oil content) groups. All these mice were fed for 5 consecutive weeks. The general status, body weight, food consumption, blood lipid indexes including total cholesterol (TC), triglyceride (TG) and high-density lipoprotein (HDL) and the weights of the visceral organs including heart, liver, spleen, lung and kidney of mice were measured once a week during the period of feeding. The results indicated that the erucic ac