狂犬病抗体免疫金标检测试纸的研制-Core.PDF

2009 8 11 4 hin J NatM ed, August2009, Vol11No4 % 241% 1 2 2 1 3 2 黄江山 , 张孟璋 , 陈仲德 , 黄亚萍 , 张长弓 , 黄印尧 , , , , () , ; , 15 min ELISA, , ! ∀ , , ; ; : R51299 : A : 1008- 7850 ( 2009) 04- 0241- 04 Study of ColloidalG old Strip in D ete ting theA ntibody of Rabies * HUANG Jiang- shan , ZHANG M eng- zhang, HEN Zhong- de, et al (* The F irstH osp ita l of X iam en, Fuj ian M ed ica l Un ivers ity, X iam en, F uj ian 361003, Ch ina ) Abstra t Obje tive To develop the colloidal gold strip in detecting the antibody of rabies M ethods W ith spe cial product technics, colloidalgold stripwas prepared and applied to detect the anti- rabies antibodyRabies antigen and anti- rabies antibody w ere coated on the test region and controlzone, respectively Results The colorw as appeared on the test re gion because of the compound of antigen- antibody w hen the sample w as positiveThere w as no color appearedwhen the sample w as negativeThew hole expermi entation merely needed fifteen minutesThe test result show ed this strip had the same merits as thatof ELISA test inm icroanalysis, high specialty and accuratyNo special instrumentwas needed and the test could be comple ted easily in a short tmi e Con lusion The colloidalgold strip in detecting the antibody of rabies based on the principle of col loidal gold mi muno- chromatography, can detect the antibody of rabies truly when the sample is positiveAnd this strip also can determ ine the content of antibody b