大豆脂肪氧合酶的提取及影响酶活因素的研究-林产化学与工业.PDF

24 2 Vol. 24 No. 2 2004 6 Chemistry and Industry of Forest Products June 2004 * 蔡 琨, 方 云 , 夏咏梅, 石玉刚, 徐一铭 ( 江南大学 化学与材料工程学院, 江苏无锡214036) CAI K : ( LOX) , 7 5 , , 10 g 6.71 10 U, 8. 61 10 U/ mL , 55% ~ 60% - - LOX 7 , 9. 42 10 U/ g LOX , 7 d 79% H 7~ 9 LOX , H 9 , LOX 1% 42 d 80% , 50 60 min 6% : ; ; :TQ91; Q814. 2 :A :0253- 2417(2004) 02- 0052- 05 EXTRACTION OF SOYBEAN LIPOXYGENASE AND FACTORS AFFECTING ITS ENZYME ACTIVITY CAI Kun, FANG Yun, XIA Yongmei, SHI Yugang, XU Yiming ( School of Chemical and Material Engineering , Southern Yangtz e University , Wux i 214036, China) Abstract: Li oxygenase( LOX) can catalyze oxygenation of the(Z, Z)1, 4 entadiene moiety of olyunsaturated fatty acids into enantiomeric hydro eroxide fatty acids with( Z, E)conjugated diene moiety. In this a er, several methodswere used for extracting LOX from defatted soybean owder, and LOX activity was measured. Ex eriment results show that the total enzyme 7 activity 6.71 10 U/ 10 g( defatted soybean flour) obtained by waterleaching is the highest with corres onding s ecific en 5 zyme activity 8. 61 10 U/ mL( enzyme re aration) .The loss of enzyme activity is more than 1/ 2 after two saltingout ste s