福建荔枝(Litchi+chinensis+Sonn.)种质资源的ISSR分析.pdfVIP

福建农林大学硕士论文 5 dNTPand of54,--56Cineachreaction polymerase，0．1I．tmol／L annealingtemperature to aS min of the arefollows．5 steps system20“L．Accordingsystem，theexperimental at secondsdenaturationat seconds predenaturation94C，30 94C，60 annealing，90 secondsextensionat72(2．The timesandendsin7minextention cyclerepeatsforty at72℃． 11 Litchifrom96 3．Atotalof ISSR were on78individualsofcultivated primersemployed 127scorable were which1 primers，and amplifiedpolymorphicdetected，of 22(96．06％)were loci．Andthis豫searchfoundthat sequence polymorphic primerscontaining(AG)n，(CA)n，(AC)n resultedinof of is amplifiedpolymorphismLitchi．So(TC)n，(GT)n，(TG)nsequencehigher levelsinLitchi genome． tothe ofISSR 78 were analysis results，thesssamples 4．Accordingclustering thatthe indexWaS0．4540．985．Thebasisof clustered，itproved geneticsimilarity for that： ISSR thetrialof78varietiesofcluster resultsshowed analysis analysis theminthe coefficientbetween among geneticsimilarity 0．454～0．985，thegenetic and betweendifferent are diversity，geneticsimilaritygeneticrelationship species itisatheoreticalbasisofdistant varieties revealed，a