混合花粉授粉茶树杂交F_2c1_代AFLP分析与优株选择.pdf

Abstract In bushesofthe this froma single Fiprogenies paper,79 mixed-polleneross(every kindof wasmixed 1980andtheir pollen equally)in usedasthe Fudingdabaicha,Baihaozao，Lungjin943)wereexperimental materials．They wereresearched byAFLP(AmplifiedFragmentLength markersand bushselectionmethodsoftea mainresultsWeleas single breeding．111e follows： 1．TheresultsshowedthatthemethodofAFLPmolecularmarker issuit technique for identification CROSS andcouldbe well． ofmixed-pollen parentage progenies repeated The79 bushesofthis couldbe Ft the single progenies distinguished by E0／M0andthefiveselective pre—amplificationprimerpairs amplieationprimer pairs (E41／M42，FAl／M55，E42／M34，E43／M42，E44／M33)． 2．BasedonNei’S distances，Fiweredividedintofour genetic progenies groupsby Pair Method、ⅣitllArithmetic DPS UPGMA(UnweightedGroup Average)of 2000．Thereare37ofthe whose are FIprogeniespatemaisZhuyeqi：8、9、11、12、 15 oftheFl whose are progeniespatemals 60、62、63、64、73．Ot