TNF拨猎谌焉锲谔悄虿⊥庵苎单个核细胞和胎盘组织中的表达研究.docxVIP

TNF拨猎谌焉锲谔悄虿⊥庵苎单个核细胞和胎盘组织中的表达研究 　　【摘要】 　　目的 研究妊娠期糖尿病（GDM）孕妇血清中TNF拨僚ǘ扔胍鹊核氐挚沟墓叵导癟NF拨 mRNA在GDM孕妇外周血单个核细胞（PBMC）和胎盘组织中的表达状况。方法 用酶联免疫吸附（ELISA）法检测空腹血清中TNF拨僚ǘ龋用Real瞭ime RT睵CR法检测PBMC和胎盘组织中TNF拨 mRNA的表达水平。结果 GDM组血清TNF拨僚ǘ让飨愿哂谡常糖耐量（NGT）组；在胎盘组织中，GDM组TNF拨 mRNA表达水平明显高于NGT组；在PBMC中，两组TNF拨 mRNA表达水平无明显变化。结论 血清TNF拨僚ǘ扔胍鹊核氐挚钩收相关，GDM组血清中升高的TNF拨量赡苤饕来源于胎盘组织，且参与了GDM 的发病过程。　　【关键词】 妊娠期糖尿病 肿瘤坏死因子 胰岛素抵抗　　【Abstract】Objective To study the relationship between the serum tumor necrosis factor alpha (TNF拨) concentrations and insulin resistance;Then detect the expression levels of TNF拨 mRNA in peripheral blood mononuclear cells and placental tissue of patients with gestational diabetes mellitus(GDM).Methods Serum TNF拨 concentrations were measured by expression levels of TNF拨 mRNA in peripheral blood mononuclear cells and placental tissue of patients with GDM were determined by Real瞭ime RT睵CR. Results The concentrations of serum TNF拨 in GDM group are markedly higher than that in normal glucose tolerance(NGT) group. The expression levels of TNF拨 mRNA in placental tissue of GDM group were obviously higher than that in NGT group,while there were not significant difference in peripheral blood mononuclear cells of both group. Conclusion The concentrations of serum TNF拨 were positively correlated with insulin resistance, the increase in circulating TNF拨 of GDM group might originate from placental tissue and contribute to the pathogenesis of gestational diabeties mellitus.　　【Key words】gestational diabetes mellitus；tumor necrosis factor；insulin resistance　　妊娠期糖尿病（gestational diabetes mellitus,GDM）发病率呈逐年上升的趋势，严重危害了妊娠母婴的健康［1］。研究表明，孕妇体内胎盘激素和脂肪细胞因子（adipocytokines）的浓度改变所造成的胰岛素抵抗（IR）是引起GDM的主要原因，遗传因素在其中发挥了重要作用。肿瘤坏死因子α（TNF拨粒┳魑一种重要的脂肪细胞因子，目前已成为GDM发病机制研究的热点之一［2］。本文应用ELISA法和Real瞭ime RT睵CR法分别对晚孕GDM母体外周血中TNF拨僚ǘ取⒛柑逋庵苎单个核细胞(PBMC)和胎盘组织中TNF拨 mRNA的含量进行了检测和分析，以期为探讨TNF拨劣胍鹊核氐挚沟墓叵担及其在GDM发病中的作用提供实验依据。　　资料和方法　　1.研究对象　　选取XX年4月至XX年1月在我院定期产检并住院分娩的孕妇，于孕24～28周行50 g糖筛查，服糖后1 h血糖≥ mmol/ L者行75 g OGTT 试验，参照乐杰《妇产科学》第6版GDM诊断标准。随机选取28例GDM孕