瓜类细菌性果斑病菌及辣椒细菌性斑点病菌的快速检测技术分析-植物病理学专业论文.docxVIP

瓜类细菌性果斑病菌及辣椒细菌性斑点病菌的快速检测技术研究 瓜类细菌性果斑病菌及辣椒细菌性斑点病菌的快速检测技术研究 watermelon infected by Aac. This method is more sensitive than that of GICA and can effectively reduce the false positive of the strip. During the assay, PCR showed a clear band even when the test line of the strip that could not be observed clearly by visual. Therefore, this method is worth of being applied for the detection of bacterial fruit blotch. (2) TaqMan real-time PCR has been widely used for the detection of bacteria because it’s more specific and sensitive than traditional PCR assay. This technique can be used to measure PCR product accumulation through a dual-labelled probe. In this research, we designed a pair of primers XCV-F-F/R and a probe XCV-F-P based on the rhs family gene for the identification of the Xcv which infects pepper only using TaqMan real-time PCR. The result showed that the TaqMan real-time PCR was 10× more sensitive and specific than that of traditional PCR assay. We also detected Xcv in leaves of inoculated pepper and washing solution of the seeds soaked in Xcv suspension. Therefore, the real-time PCR method in this study is rapid and reliable for the detection of Xcv on pepper in the field. The GICA-PCR and TaqMan real-time PCR in this research were sensitive and specific for the rapid and accurate detection of the target bacteria. These two methods solved the problems of the GICA and traditional PCR assay respectively. Both assays are significant for the safety of the environment and the import and export trade in our country. Keywords: bacterial fruit blotch; bacterial spot disease; rapid detection; GICA-PCR; real-time PCR 4 关于学位论文原创性和使用授权的声明 本人所呈交的学位论文，是在导师指导下，独立进行科学研究所取得 的成果。对在论文研究期间给予指导、帮助和做出重要贡献的个人或集体， 均在文中明确说明。本声明的法律责任由本人承担。 本人完全了解山东农业大学有关保留和使用学位论文的规定，同意学 校保留和按要求向国家有关部门或机构送交论文纸质本和电子版，允许论 文被查阅和借阅。本人授权山东农业大学可以将本学位论文的全部或部分 内容编入有关数据库进行检索，可以采用影印、缩印或其他复制手段保存 论文和汇编本学位论文，同时授权中国科学技术信息研究所将本学位论文 收录到《中国学位论文全文数据库》，并向社会公众提供信息服务。 保密论文在解密后应遵守此规定。 论文作者签名： 导 师 签 名： 日 期： 山东农业大学硕士学位论文 山东农