离子束介导遗传转化小麦突变体的遗传分析-河南农业科学.PDF

DOI :10.15933/ki.1004 -3268.2010.05.024 河南农业科学 1 1 1 2 3 1 王铁固, 杨天佑,赵新亮 ,冯伟森 ,黄群策 , 陈士林 (1. , 453003;2., 471022; 3. , 450052) :以离子束介导后代的9 个小麦突变体和4 个受体作供试材料, 对其进行了RAPD 和SSR分 子标记,在 基础上获得了矮秆突变株系 1042 的2 个特异片断, 并进行了测序和同源序列分析。 13 个材料间分子标记遗传差异比较结果表明, 离子束介导遗传转化后的突变明显小于小麦品种间 的遗传差异。矮秆突变株系1042 的SSR标记特异片断序列BLAST 分析结果表明,从 1042 中扩 增到的特异片断与小麦高秆基因R h t-D1a 中的部分序列高度 一致, 1042 的株高明显降低可能是由 于该株系遗传突变, 产生了2 个矮秆基因所致;该特异片断与小麦BA 克隆中LT R-retrotranspo- son Angela-3s 转座子的部分片断同源性最高,说明离子束介导遗传转化诱发变异可能与小麦基因 组中反转录转座子的激活有关。 :小麦;离子束;遗传转化;RAPD;SSR;序列分析 :S512.1　　:A 　　:1004-3268(2010)05-0011-05 Genetic Analysis of Wheat Mutants by Ion Beam-mediated T ransformation 1 1 1 2 WANG Tie-gu ,YANG Tian-you ,ZHAO Xin-liang ,FENG Wei-sen , 3 1 HUANG Qun-ce , HEN Shi-lin (1.School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang, 453003, hina; 2.Agricultural Science Institute of Luoyang, Luoyang 471022, hina;3.Henan Provincial Key Laboratory of Ion Beam Bio-engineering,Zhengzhou University, Zhengzhou 450052, hina) Abstract:Nine wheat mutants from genetic transformation mediated by ion beam and their 4 re- cipient varieties were taken as test materials to conduct RAPD and SSR marker analysis, and then 2 special fragments from the dwarf mutant 1042 were sequenced, homology analysis of which was done.The results showed that the variance of mutants from genetic transformation byt ion beam was smaller than the genetic variance between different recipient varieties.After BLAST analy- sis, th