钩藤碱对帕金森大鼠脑内SOD、DA、MDA表达的调节作用.docxVIP

钩藤碱对帕金森大鼠脑内SOD、DA、MDA表达的调节作用 王向明陆学胜 (上海市交通大学医学院附属同仁医院200336) 【摘要】目的 探讨钩藤碱在帕金森病(PD)大鼠模型中对纹状体多巴胺(DA)、 超氧化物歧化酶(SOD)、丙二醛(MDA)水平表达的调节作用。方法 首先从中药钩 藤中分离提取出钩藤碱。其次釆用脑内定位注射6-OHDA制备部分损伤PD大鼠 模型，采用正常对照组、模型对照组和钩藤碱组来进行分析。用牛化法对大鼠脑 中DA、及血清中SOD、MDA活性进行测定，分析钩藤碱对其活性的影响。结果 给药30天后，钩藤碱组DA水平密度明显高于模型对照组(Plt;0.01)o在SOD水 平方面，钩藤碱组显著高于模型组(Plt;0.05)；而MDA含量与正常对照组比较, 而钩藤碱组与正常对照组接近，显著低于模型对照组组(Plt;0.05)o结论钩藤 碱对帕金森大鼠脑内SOD、DA、MDA表达的具有调节作用，提示其作为帕金森 症的治疗或者预防药物具有一定的开发价值。 【关键词】钩藤碱 帕金森 大鼠 【中图分类号】R96 【文献标识码】A 【文章编号】2095-1752 (2014) 14-0028-02 The expression level adjustment of rhynchophylline on Dopamine, superoxide dismutase and malondialdehyde in Parkinson disease (PD) rats [Abstract] Objective To study rhyn chophylli ne in Parkins on disease (PD) in rats model on Dopamine(DA), superoxide dismutase (SOD) and malondialdehyde (MDA) expression level adjustment. Method First to extracte rhynchophylline from traditional Chinese medicine uncaria. The second part is to injury PD rat model by Brain positioning injection of 6-OHDA, compare and analyze normal group, model group and rhynchophylline group. Determine DA, SOD in serum and MDA activity in ratsrsquo; brains by biochemical method, and analyze the impact of rhynchophylline on its activity. Results After 30 days drug administration, DA density level of rhynchophylline group was significantly higher than that of model group (Plt;0.01). At the SOD level, rhynchophylline group was sign讦icantly higher than that in model group (Plt;0.05). Mean while the content of MDA compared with the normal group, rhynchophylline group and normal group ware close and significantly lower than that of model group (Plt;0.05). Conclusion Rhynchophylline adjust SOD, DA and MDA in Parkinson rat srsquo; brains. It shows a value of development in Parkinsorfs disease treatment or prevention of drug? 【Key words］ rhynchophylline Parkinson rats 帕金森病，是j种中老年人常见的运动障碍疾病,以黑质多巴胺能神经元变性 缺失和路易小体形成为病理特征，以静止性震颤、肌强直、运动迟缓和姿势步态 异常为主要临床特征［1］。目前，西药疗法在帕金森治疗中处于核心地位，药物美多 巴因效果显著被公认为“金药物”，虽然仍然无法阻止帕金森病自然发展的进程