从农作物的残余物中萃取纯化阿魏酸与其功效-廖婕妤.DOC

國立宜蘭大學食品科學研究所 碩士班專題討論 演講者：廖婕妤 (Liao, Jie-Yu) 題目：從農作物的殘餘物中萃取純化阿魏酸與其功效評估 指導教授：陳翠瑤 老師 The extraction and purification of ferulic acid from 日期：Nov. 23, 2011 crop residues and the evalution of physiological activity 中文摘要 阿魏酸是一種普遍存在植物中的酚酸類化合物，主要存在穀類的糠、柑橘類、香蕉、咖啡，具有生理活性。阿魏酸具抗氧化能力及預防或治療阿茲海默症、糖尿病、高血壓、動脈硬化等疾病。許多農作物的殘餘物中皆含有阿魏酸，可由亞麻桿、小麥糠、玉米糠、甘蔗渣等萃取純化出阿魏酸，增加殘餘物的再利用。利用鹼水解及加壓低極性水萃取技術可取得阿魏酸，可利用活性碳吸附以及大孔陰離子交換樹脂進行分離純化得到高純度的阿魏酸。阿魏酸可抑制黃嘌呤氧化酶、NADPH氧化酶的活性，達到保護腸道受到缺血後灌流 (I / R)的損傷，可以用在治療各種器官中，因血液再灌注過程引起的缺血性疾病。另外阿魏酸可抑制血管加壓素Ⅱ所誘導的血管平滑肌細胞的增生作用，具有顯著的效果並且有劑量依存性，可以預防動脈硬化等心血管疾病。 關鍵字:阿魏酸、萃取、純化、生理活性 Abstract Ferulic acid (FA) is a ubiquitous phenolic compounds in plants, primarily in grain bran, citrus fruits, bananas and coffee with bioactive physical activities. FA has antioxidant and may be beneficial in prevention and/or treatment of Alzheimer’s disease, diabetes, hypertension and atherosclerosis. Crop residues contain ferulic acid flax shives, wheat bran, corn bran and sugarcane bagasse. Extraction FA from that, can increase the use of resources. Ferulic acid can be extracted by using alkaline hydrolysis and pressured lowered polarity water, and obtain high-purity of FA , by activated charcoal adsorption/anion macroporous resin exchange chromatography. Ferulic acid could inhibit the activities of xanthine oxidase, NADPH oxidase, protection from ischemia reperfusion (I / R) injury, that could apply on the limitation the damage induced by the reperfusion process in the treatment of ischaemic disorders in various organs. It was shown that ferulic acid significantly inhibited angiotensin II-induced VSMC proliferation in a dose-dependent manner,that would be prevention atherosclerosis. Keywords: Ferulic acid, extraction, purification, bioactive References * Buranov, A. U., Mazza, G. (2009). Extraction and purification of ferulic acid from flax shives, wheat and corn bran by alkaline hydrolysis and pressurised solvents. Food Chemistry, 115: 1542–1548.(IF 3.458;Chemist