内蒙古荞麦总黄酮含量测定.pdfVIP

1415ls02-05 52 粮食与油脂 2015 年第 28 卷第 2 期 内蒙古荞麦总黄酮含量测定 闫　超，郭　军，张美莉，刘莉敏 （内蒙古农业大学食品科学与工程学院，　内蒙古呼和浩特　010018 ） 摘　要：从内蒙古和其它省区采集了37 种荞麦，75% 乙醇回流提取后用分光光度法测定总黄酮含 量。结果表明，总黄酮含量（均值±2SD ，单位：mg/kg ）分别为，内蒙甜荞409.61 ±287.07 （N =23 ）， 其中一个内蒙甜荞样品总黄酮含量（3 553.42 ）极高未计入均值，其它省区甜荞503.51 ±411.29 （ N =7 ），差异不显著；实验室自去皮甜荞样品524.27 ±340.81 （N =16 ），高于无皮甜荞样品 （325.52 ±85.56 ，N =14 ），差异极显著（ρ=0.000 ）；苦荞总黄酮含量（16 177.23 ±1841.40 ，N =6 ）为 甜荞（431.52 ±322.56 ，N =30 ）的37 倍。测定结果为鲜有报道的内蒙古荞麦总黄酮含量提供了数据。 关键字：内蒙古；荞麦；总黄酮；分光光度法 Determination of total flavonoids content of Inner mongolia buckwheat YAN Chao ，GUO Jun ，ZHANG Mei-li ，LIU Li-min （College of Food Science and Engineering ，Inner Mongolia Agricultural University ，Huhhot 010018 ，Inner Mongolia ，China ） Abstract ：37 kinds of buckwheat samples from Inner Mongolia and other regions were collected. Total flavonoids were extracted by 75% ethanol and determined by spectrophotometry. Results showed that total flavonoids content （mean ±2SD ，mg/kg ）of Inner Mongolia common buckwheat was 409.61 ±287.07 （ N =23 ）. And content of other regions common buckwheat was 503.51 ±411.29 （N =7 ）. The difference was not significant. One sample of Inner Mongolia common buckwheat ，with high total flavonoids content （3 553.42 ），was not included in the average. Content of common buckwheat samples which were peeled in laboratory was 524 .27 ±340.81 （ N =16 ） and higher than hulless samples （325.52 ±85.56 ， N =14 ）. The difference was significant （ρ=0.000 ）. Tartary buckwheat （16 177.23 ±1 841.40 ， N =6