贯叶连翘提取工艺及质量控制体系的构建-植物学专业论文.docxVIP

(4)贯叶连翘提取物指纹图谱的初步研究。采用高效液相色谱技术对贯叶 (4)贯叶连翘提取物指纹图谱的初步研究。采用高效液相色谱技术对贯叶 连翘药材和提取物的色谱指纹图谱进行研究，并采用高效液相色谱一质谱技术对 贯叶连翘药材和提取物中的成分进行归属。在上述基础上初步确定贯叶连翘药材 的特征指纹峰，并选取17个成分作指标，考察了在10批提取物的色谱指纹图谱 之间上述17个成分相对保留时间的一致性、相对峰面积的一致性：采用直观分 析法对10批贯叶连翘提取物的色谱图作直观比较，考察10批提取物指纹图谱的 相似性、主要成分保留时间和峰面积相对比例的一致性；并采用相似度计算软件 计算各指纹图谱的相似度。结果表明，所建立的贯叶连翘药材和提取物质量的色 谱指纹图谱分析方法具较好的稳定性、重现性和普遍适用性，所建立的贯叶连翘 药材和提取物的指纹图谱可为贯叶连翘药材和提取物的质量控制提供更全面的 信息。 关键词：贯叶连翘，提取工艺，质量标准，指纹图谱，金丝桃素，贯叶金丝桃素， 总黄酮 Ⅱ Study Study on Extracting Techniques of Hypericum perforatum厶and Quality Control System Major：Botany(Natural Pharmaceutical Chemistry) Name：Liu chao．shen Supervisor：Prof．Yang De-po Co··Supervisor：Associate Prof．Wang Dong·rnei ABSTRACT Hypericum perforatum L a herbal that was famous in the world for its antidepressant activity,has got more and more domestic and external attentions since 1980s．Newly research showed that Hypericum perforatum厶possesses a lot ofefficiency such嬲antivirns，anticencer,antibiotic，diminish inflammation and modulating inordinate menstruation．The main chemical constituents ofthe plan a∞naphthodientrones，flavonoids and pMomglucinoB．In the late of 1980s，it Wns found this plant can inhibit HIV evidently,which made it become focus again．In old朗to find and develop effectual antidepressant and entiviral medicine from this plant,the following∞search had been done by US (1)Review．Thoroughly review the studies of the plant on phytochemistry, pharmacology,clinical application,untoward reaction,extracting techniques and quality standard at home and abroad． (2)Multi-indices analysis of the previous extracting techniques．Ten constituents were chose粥index to study the enrichment degree of the main effectual constituents,the reproducibility and stability of the extracting techniques，the affection of anfiomdant and medicinal materials on the constituents ofthe extractive。 (3)Optimization of the extracting techniques．We used hypedcins， Ⅲ hyperforin hyperforin and flavanoids髂index to optimize the extractive temperature．use level