大孔树脂纯化茶皂素工艺研究.pdfVIP

2014年第39卷第11期　 　 　 　 　 　 　 　 　 　 　 　 　 中　 国　 油　 脂 69 综合利用 大孔树脂纯化茶皂素工艺研究 张云丰,汪立平 (上海海洋大学食品学院,上海201306) 摘要:采用大孔树脂对茶皂素粗品进行分离纯化。 以茶皂素得率为指标,采用响应面法优化洗脱条 件。 通过考察不同极性的大孔树脂D-101、AB-8、S-8、X-5、HPD-100 的吸附与解吸性能,从 中筛选出具有代表性的S-8 大孔树脂,并对其吸附和解吸能力进行综合分析。 确定S-8 大孔树 脂纯化茶皂素最佳工艺条件为:S-8 大孔树脂装柱量100 g,质量浓度为0.703 mg/ mL的茶皂素溶 液110 mL,上样流速3.03 mL/ min,洗脱剂乙醇体积分数90%,乙醇流速3.03 mL/ min,乙醇用量 100 mL。 在最佳条件下,对含量为47.38% 的茶皂素粗品进行分离纯化,最终茶皂素得率为 81.74%,回收率为78.29%,含量为85.36%。 关键词:茶皂素;大孔树脂;吸附;解吸;纯化 中图分类号:TS229;TQ423.3　 　 文献标志码:A　 　 文章编号:1003-7969(2014)11-0069-05 Purification of tea saponin by macroporous resin ZHANG Yunfeng,WANG Liping (College of Food Science and Technology,Shanghai Ocean University,Shanghai201306,China) Abstract:The crude tea saponinwas separated and purified by macroporousresin. Using theyield of tea saponin as indicator, the elution conditions were optimized by response surface methodology. S - 8 macroporous resin was screened out by studying the adsorption and desorption properties of macroporous resins D-101,AB-8,S-8,X-5 and HPD-100. The optimal adsorption conditions were obtained as follows: dosage of S - 8 macroporous resin 100 g, mass concentration of tea sapoin solution 0.703 mg/ mL,loadingvolume 110 mLandflow rateof sample3.03 mL/ min,volumefractionof ethanol (eluent)90%,flow rate 3.03 mL/ min,dosage of ethanol 100 mL. After 47.38% of tea saponin was separated and purified under the optimal conditions,the yield,recovery rate and content of tea saponin were 81.74%,78.29% and 85.36%,respectively. Key words:tea saponin;macroporous resin;adsorption;desorption;purification 　 　 茶皂素是山茶科山茶属植物皂素的统称,是一 中,难溶于冷水、无水乙醇,在稀碱性水溶液中溶解 类齐墩果烷型五环三萜类皂苷化合物,由亲水性糖 性显著增加,不溶于乙醚、氯仿、石油醚及苯等溶剂。 基和疏水性苷元及有机酸组成[1-2]。 茶