大豆分离蛋白在尿素∕无机盐水溶液中的变性与流变特性.pdfVIP

2014年第39卷第5期摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 摇 中摇 国摇 油摇 脂 33 油料蛋白 大豆分离蛋白在尿素/ 无机盐水溶液中的 变性与流变特性 郭子民,刘摇 思,吴海燕,王摇 旭,尹翠玉 (天津工业大学材料科学与工程学院,改性与功能纤维天津重点实验室,天津300387) 摘要:将大豆分离蛋白溶解在尿素/ 无机盐Au 水溶液中,研究尿素/ 无机盐Au 水溶液体系中尿素 的浓度和无机盐Au加入量对大豆分离蛋白的变性和流变特性的影响。 结果表明:大豆分离蛋白 在尿素/ 无机盐Au水溶液体系中能够形成稳定且流动性良好的溶液;大豆分离蛋白在尿素/ 无机 盐Au水溶液中结构被破坏,溶解性增加;溶液表观黏度随无机盐Au加入量增加先减小后增加,随 尿素浓度的增加明显下降,随温度升高而降低;大豆分离蛋白尿素/ 无机盐Au 水溶液的吸光度随 着尿素浓度的增加明显减小,而无机盐Au加入量对大豆分离蛋白尿素/ 无机盐Au 水溶液的吸光 度的影响不明显。 关键词:大豆分离蛋白;脲变性;流变特性 中图分类号:TS229;TQ936摇 摇 文献标志码:A摇 摇 文章编号:1003-7969(2014)05-0033-04 Denaturation and rheological properties of soybean protein isolate in urea/ inorganic salt solution GUO Zimin,LIU Si,WU Haiyan,WANG Xu,YIN Cuiyu (1. Tianjin Key Lab of Fiber Modification Function,School of Materials Science and Engineering,Tianjin Polytechnic University,Tianjin300387,China) Abstract:The soybean protein isolate was dissolved in urea/ inorganic salt Au aqueous solution,and the effects of urea concentration and inorganic salt Au dosage inurea/ inorganic salt Au aqueoussolution sys鄄 tem on denaturationandrheologicalpropertiesof soybeanproteinisolatewerestudied. Theresultsshowed thatinurea/ inorganicsaltAuaqueoussolutionsystem,soybeanproteinisolatecouldformstableandgood fluidity solution and its structure was destroyed,making its solubility increase;with inorganic salt Au dosage increasing,the apparent viscosity of the solution first decreased and then increased,while it re鄄 duced significantly with urea concentration increasing and temperature rising;the absorbance of urea/ in鄄 organic salt Au aqueous solution of soybean protein isolate declined markedly with urea concentration in鄄 creasing,while inorganic salt Au dosage had no obvious effect