产纤维素酶食用菌筛选及其培养基的优化.PDFVIP

236 2012, Vol.33, No.21 食品科学 ※生物工程 丁玉萍，张 康，叶红强，王晓娟，杨 莎，梅 竹，任欢欢 (佳木斯大学生命科学学院，黑龙江 佳木斯 154007) 摘 要：利用刚果红杯碟快速筛选法对大白菇、金针菇、香菇、竹荪、磷盖红菇、杏鲍菇6种食用菌产纤维素酶能 力进行比较，同时对高产纤维素酶食用菌的培养基最佳碳源和配方进行筛选、优化。结果表明：磷盖红菇出现透明 圈最大，直径为20mm ；对比羧甲基纤维素钠(CMC-Na)、豆渣、秸秆粉、葡萄糖4种碳源，豆渣对磷盖红菇产纤维 素酶有促进作用，优化培养基为豆渣60g/L、硫酸铵2.0g/L 、吐温-80 1.5mL/L、pH5.4 。 ：食用菌；纤维素酶；筛选；培养基 Screening for Cellulase Producing Edible Fungi and Optimization of Medium DING Yu-ping ，ZHANG Kang ，YE Hong-qiang ，WANG Xiao-juan ，YANG Sha ，MEI Zhu ，REN Huan-huan (College of Life Science, Jiamusi University, Jiamusi 154007, China) Abstract ：The ability of six edible fungi, Russula delica Fr , Flammulina velutipe, Lentinus javanicus , Bamboo-sun , Russula lepida and Abalone mushroom, to produce cellulase were compared using the Congo red saucer rapid screening method. Meanwhile, the optimum carbon source and medium formula for cellulase production were screened and optimized. The result showed that Russula lepida produced the largest transparent rings of 20 mm in diameter, showing its high yield of cellulase. Among the four kinds of carbon source, CMC-Na, bean dregs, straw powder and glucose, the bean dregs promoted Russula lepida to produce cellulase. The optimized medium for cellulase production by Russula lepida was bean dregs 60 g/L, ammonium sulfate 2.0 g/L, Tween-80 1.5 mL/L, and pH 5.4. Key words ：edible fungi ；cellulase ；screening；medium 中图分类号：Q939.99 文献标识码：A 文章编号：1002-6630(2012)21-0236-04 食用菌在分类学上属于菌物界真菌门，是一类可以食 1号：大白菇；2号：金针菇；3号：香菇；4号：竹 用的大型真菌[1-2] 。食用菌栽培所用碳源主要是锯末、秸秆 荪；5号：磷盖红菇；6号：杏鲍菇；菌种由佳木斯大学 等富含纤维素、半纤维素、木质素的物质，食用菌分泌的