胍丁胺对面神经损伤修复作用及机制的实验初探-耳鼻咽喉科学专业论文.docxVIP

重庆医科大学硕士研究生学位论文 重庆医科大学硕士研究生学位论文 胍丁胺对面神经损伤修复作用及机制的实验初探 摘 要 目的 以SD雄性大鼠面神经横断端端吻合模型，研究胍丁胺对面 神经损伤修复再生作用的影响，并探索其可能作用机制。 方法48只SD雄性大鼠随机平均分为正常组、对照组、胍丁胺 治疗组。建立大鼠双侧面神经主干横断伤，10—0无损伤线行神经外膜 吻合。术后治疗组予以胍丁胺1 00mg／kg腹腔注射7d后，对照组则予 以0．9％生理盐水注射，正常组不予任何处理。将每组动物随机平均分为 两组：一组在术后2w用ELISA法检测其脑干面神经核团部分的脑源 性神经营养因子浓度；另一组在术后4w取吻合处远心端再生面神经段 3-4mm，Luxol坚牢蓝染色，显微镜下观察有髓轴突比率和轴突厚度。 结果48只动物全部进入结果分析。术后2w，对照组及胍丁胺治 疗组大鼠脑干面神经核团部位脑源性神经营养因子的浓度显著高于正 常组，其中胍丁胺治疗组亦明显高于对照组，组间差异均有显著性。 术后4w，对照组髓鞘可见大量的空泡变性，部分轴突变性缺失，再生 轴突少。胍丁胺治疗组可见再生轴突已经通过切断处，轴突变细，排 列较整齐，有一定数量的髓鞘形成，雪旺细胞增生明显，轴索间水肿 减轻。但两组较之正常组神经束面积及轴突数目均减少，组间差异均 有显著性。 结论外源性胍丁胺可促进面神经损伤后修复再生，这一作用可 3 重庆医科大学硕士研究生学位论文能与其可增加脑干面神经核团部位脑源性神经营养因子的浓度有关。 重庆医科大学硕士研究生学位论文 能与其可增加脑干面神经核团部位脑源性神经营养因子的浓度有关。 关键词：面神经，胍丁胺，脑源性神经营养因子，酶联免疫吸附测 定 4 重庆医科大学硕士研究生学位论文PRELIMINARY 重庆医科大学硕士研究生学位论文 PRELIMINARY EXPERIMENTAL STUDY ON RoLE AND MECHANISM OF AGMATINE IN REGENERATIoN OF FACIAL NERVE I NJURY ABSTRACT objective To observe the effecting and presume the mechanism of agmatine in regeneration of facial nerve injury． Methods 48 SD male rats were randomly and evenly divided into normal，control and Agm treatment groups．The bilateral facial nerve main trunks were transected．Coaptation of the proximal and distal stumps of the cut nerve was achieved by suturing the epineurium of nerve using a 1 O-Onylon thread under the operating microscope．The Agm treatment group was peritoneal inj ected with 1 00mg／kg agmatine once a day for a week．Saline—injected rats served as controls．The normal group was untreated．Then each group was randomly and evenly divided into two parts． At the 2 weeks，concentrations of Brain—derived Neurotrophic Factor in brain—stems were observed by Enzyme Linked Immunosorbent Assay；at the 4 weeks after operation，about 3-4mm distal regenerated nerve segment was dissected，cut into sections．The sections were processed for staining using Luxol fast blue，then observed the number of axis—cylinder and area of nerve tract on microscope equiped with a video camera connected to 5 重庆医科大学硕士研究生学位论文mor