在AOT异辛烷反相胶束体系中酶法合成RGD前体二肽.PDFVIP

第 15 卷第 1 期 中国生物化学与分子生物学报 V o l. 15,N o. 1 1999 年 2 月 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy Feb. 1999 ·研究简报· 在AO T异辛烷反相胶束体系中酶法合成RGD 前体二肽 陈育新　张学忠 　陈松明　吴晓霞 ( 吉林大学酶工程实验室, 长春 130023) Enzyma tic Syn thesis of Precursor D ipeptides of RGD in AOT Isooctane Reverse M icellar System , , , CH EN Yux in ZHAN G Xuezhong CH EN Songm ing W U X iaox ia ( , , 130023) N ational L aboratory of E nzym e E ng ineering J ilin U niversity Chang chun 　 , Abstract T he p recu rso r dipep tides of R GD Boc A rg Gly O E t and A c Gly A sp diOM e w ere syn . thesized in AO T isooctane reverse m icellar system u sing differen t p ro teases T he reaction condi tion s fo r the Boc A rg Gly O E t syn thesis catalyzed by alcalase and fo r A c Gly A sp diOM e syn the sis catalyzed by tryp sin in AO T isooctane reverse m icelles w ere op tim ized by exam in ing the ef fects of several facto rs including w ater con ten t, tem peratu re, pH and reaction tim e on the dipep . tide yields T he best yields fo r alcalase catalyzed syn thesis of Boc A rg Gly O E t and tryp sin cat