BI274487和AA963863与大鼠再生肝8种细胞的细胞外基质代谢Three.PDFVIP

HEREDITAS (Beijing) 2011 年 ISSN 0253-9772 新基因 BM390716 、BI274487 和 AA963863 与大鼠再 生肝 8 种细胞的细胞外基质代谢 1, 2 2 1, 2 王泽 ，郭学强 ，徐存拴 1. 河南师范大学生命科学学院，新乡 453007 ； 2. 河南省-科技部共建细胞分化国家重点实验室培育基地，新乡 453007 摘要：为了解新基因BM390716 、BI274487 、AA963863 在细胞外基质代谢中的作用及其与大鼠肝再生的相 关性，文章用 Percoll 密度梯度离心结合免疫磁珠分选分离大鼠再生肝的 8 种细胞，用 Rat Genome 230 2.0 芯片检测它们的基因表达变化，用 Microsoft Excel 、BLAST 等软件分析基因的共表达关系、序列同源性及 参与的代谢活动。结果表明，BM390716 与ppar α 同源和共表达，BI274487 与 timp2 同源和共表达，AA963863 与csgalnact1 同源和共表达。根据上述基因的同源性和共表达推测，新基因BM390716 、BI274487 和AA963863 参与大鼠再生肝 8 种细胞的细胞外基质代谢。 关键词：大鼠肝再生；Rat Genome 230 2.0 芯片；基因的同源性和共表达；细胞外基质代谢 Three novel genes BM390716, BI274487 and AA963863 involed in extracellular matrix metabolism of eight rat regenerating liver cell types 1,2 2 1, 2 WANG Ze , GUO Xue-Qiang , XU Cun-Shuan 1. College of Life Science, Henan Normal University, Xinxiang 453007, China; 2. Key Laboratory for Cell Differentiation Regulation, Xinxiang 453007, China Abstract: To explore the roles of three novel genes BM390716, BI274487 and AA963863 and the correlation between them during liver regeneration of rats, eight kinds of liver cells were isolated using the combined percoll density gradient centrifugation and immunomagnetic bead method. Rat genome 230 2.0 array was used to detect the changes in expression of genes involved in metabolism of extracellular matrix and the novel genes in rat genome. Correlation between sequence homology, co-expression of the above genes and the physiological activities they involed in were analyzed using Microsoft Excel and BLAST software. The results showed that BM390716 was homologous to and co-expressed with ppar α, BI274487 was homologous to and co-expre