含水量对柱层析同时分离卵磷脂和脑磷脂影响的研究-生物化工专业论文.docx

摘要卵磷脂和脑磷脂是两种重要的磷脂，在食品乖医药等行业应用广 摘要 卵磷脂和脑磷脂是两种重要的磷脂，在食品乖医药等行业应用广 泛。高纯度磷脂由于其独特性能越来越受到人们关注。目前国内所用 高纯度磷脂产品多从国外进口，年需求量数吨以上。因此开发出适合 国情的高纯度磷脂产品迫在眉睫。本文重点考察了在卵磷脂和脑磷脂 柱层析精制过程中，固定相和流动相的含水量对此分离过程的影响。 首先，采用有机溶剂从新鲜蛋黄中粗提磷脂，最终确定工业丙酮 脱脂、工业酒精提取的工艺条件，为后续精制工艺提供了很好的原料。 其次，作者在大量的实验研究中发现硅胶自身的含水量以及流动 相的含水量严重影响柱层析同时分离纯化卵磷脂和脑磷脂的实验结 果。因此本文系统地考察了含水量对硅胶柱层析同时分离卵磷脂和脑 磷脂过程的影响。得出硅胶含水量在1 0—12％(wt)和流动相含水量在 0．5—0．9％(v01)范围内，卵磷脂和脑磷脂可实现基线分离，卵磷脂 的回收率可达85％以上，脑磷脂的回收率可达7 o％以上。 同时，作者还系统地考察了含水量对中性氧化铝柱层析同时分离 卵磷脂和脑磷脂过程的影响。得出氧化铝含水量在8％(wt)和流动相 含水量在0．1—1．1Yo(rot)范围内，卵磷脂和脑磷脂可实现基线分离， 脑磷脂的回收率可达90％以上，脑磷脂的回收率可达40％以上。 本文的研究结果除了对高纯度卵磷脂弄口脑磷脂的制备有一定的 指导作用外，对其它天然产物分离纯化的研究和生产也有一定的借鉴 意义。 关键词：卵磷脂脑磷脂硅胶中性氧化铝含水量 AbstractBoth Abstract Both Phosphatidyl Choline(PC)and Phosphatidyl Ethanolamine(PE) are iraportant sorts of phospholipids，being widely used in the filed of food，pharmacy and SO on．People pay more attention to the nature high— purity phospholipids because of its special property．At present，almost all of the high-purity phospholipids used in our country are imported，the annual demand is upward to several tons．So it is necessary to study how to produce high—purity phospholipids．The effects of water contents in stationary Phase and mobile phase on the simultaneous purification of PC and PE by column chromatography were studied systematically in this paper At first，the process condition of extraction of phospholipids from egg yolk was investigated．The process included of lipid removal process with acetone，following with extraction process with ethanol，which provided good raw material of the following purification process Then，the effects of water contents in silica—gel and mobile phase on the simultaneous purification of PC and PE by silica．gel column chromatography were studied systematically in this thesis．The results showed that the water contents in silica—gel and mobile phase had great influence on the purification process．In the range of 1 0—。1 2％(wt)for water content in silica—gel and 0．5-0．9％(v01)for water content in mo