明月草及紫背菜挥发油的研究.docxVIP

中文摘要 明月草（Angelica keiskei koidzumi）和紫背菜(Gynura bicolor DC)均为菊科菊三七属植物，在民间已被作为药材使用，富含多种营养物质及活性成分，且具有多方面的药理活性。明月草全草可入药，含有多种营养成分和天然活性物质。紫背菜也是一种可食用可入药的野菜，有较好的营养价值和保健功能。目前已有对其营养成分和粗提物的研究，但尚未有对于明月草和紫背菜挥发油的完整研究。本实验研究采用水蒸气蒸馏法提取明月草和紫背菜的挥发油，通过气相色谱-质谱（GC-MS）联用技术对两种挥发油的组成成分进行分析，结果从明月草挥发油中鉴定出8种化合物，其中含量最高的是苯酚（C6H6O），占69.294%，其次是桉叶油素（C10H18O），含量为16.732%；从紫背菜挥发油中鉴定出27种化合物，含量最高的为桉叶油素（C10H18O），占27.022%；其次是己基过氧化氢（C6H14O2），含量为18.742%。两种挥发油中共有4种相同成分，分别是：桉叶油素，Cubenol，4-萜烯醇和α-松油醇。对两种挥发油进行生物活性测试，包括抑菌活性实验、最低抑菌浓度（MIC）测试和化感活性实验。结果表明明月草挥发油对大肠杆菌、枯草芽孢杆菌、番茄细菌性溃疡病菌及绿脓杆菌均有抑菌活性，紫背菜挥发油对大肠杆菌、枯草芽孢杆菌、番茄细菌性溃疡病菌具有抑菌活性，明月草和紫背菜挥发油均对鬼针草、黑麦、稗草种子的发芽有抑制作用。 关键词：明月草 紫背菜 挥发油 GC-MS 生物活性 Abstract Angelica keiskei koidzumi and Gynura bicolor DC are compositae plants of Gynura Cass, which have been used as medicine in the folk, rich in a variety of nutrients and active ingredients, and has various pharmacological activities. The whole plant of Angelica keiskei koidzumi can be used as a medicine, containing a variety of nutrients and natural active substances. Gynura bicolor DC is also a kind of edible wild vegetables which can be used as a medicine, having good nutritional value and health care effect. At present, the research on their nutritional components and crude extract has been done, but there is no complete study on their volatile oils. Volatile oils of Angelica keiskei koidzumi and Gynura bicolor DC in this experiment were obtained by steam distillation method, and the components of volatile oils were analyzed by gas chromatography-mass spectrometry (GC-MS). 8 different compounds were identified from the Angelica keiskei koidzumi volatile oil, of which the most was Phenol (C6H6O), accounting for 69.294%, followed by Eucalyptol(C10H18O), the content was 16.732%; while 27 different compounds were identified from Gynura bicolor DC volatile oil, the most was Eucalyptol(C10H18O), accounting for 27.022%, followed by Hexyl hydroperoxide (C6H14O2), with a content of 18.742%. Two kinds of volatile oil had 4 same c