抗冷基因RLK的遗传转化研究.docVIP

吉林大学学士学位论文（设计）承诺书 本人郑重承诺：所呈交的学士学位毕业论文（设计），是本人在指导教师的指导下，独立进行实验、设计、调研等工作基础上取得的成果。除文中已经注明引用的内容外，本论文（设计）不包含任何其他个人或集体已经发表或撰写的作品成果。对本人实验或设计中做出重要贡献的个人或集体，均已在文中以明确的方式注明。本人完全意识到本承诺书的法律结果由本人承担。 承诺人 年 月 日 PAGE \* MERGEFORMAT PAGE \* MERGEFORMAT III 中文摘要 玉米是重要的粮食作物，种植范围广泛，世界三大著名玉米黄金带分别位于美国、墨西哥和中国。我国幅员辽阔，玉米种植形式多样，受地理因素影响，东北地区低温冷害频率较高，严重影响到玉米的产量和质量。随着玉米在工业和农业方面需求量的不断攀升，东北地区玉米遭受低温胁迫的问题亟待解决。 低温冷害会诱导玉米ZmRLK基因的表达，并且表达量与低温处理时间成正比。RLK是富含亮氨酸重复跨膜区域的类受体蛋白激酶，在抗非生物胁迫方面发挥重要作用，ZmRLK过表达可以增强植株对冷害的耐受能力。 本研究选用玉米自交系HiII的幼胚作为受体材料，将抗冷基因RLK用农杆菌介导法侵染转化导入幼胚，进一步培养，诱导形成胚性愈伤组织，再分化获得转化植株，进而提高玉米抗冷性，培育出能够抗冷的玉米新材料。主要研究结果如下： 从HiII玉米果穗中接种幼胚，以农杆菌介导法进行侵染，经双丙氨膦多次筛选，获得382块抗性愈伤组织，分化后共获得抗性苗177株。经PCR分子检测，其中带有Bar基因的有21棵，阳性率约为11.9%；带有特异片段35S-ZmRLK的12棵，阳性率约为6.8%。本研究利用现代生物技术对玉米进行遗传改良，为进一步提高玉米抗冷性提供实验依据。 关键词：玉米；RLK基因；幼胚；农杆菌介导法；遗传转化 Abstract Maize is an important food crop, planting a wide range. The worlds three famous maize gold belt were located in the United States, Mexico and China. Chinas vast territory, maize planting in various forms, affected by geographical factors, the northeastern region of high frequency chilling injury frequency is high, seriously affecting the yield and quality of maize. With the increasing demand for maize in industry and agriculture, the problem of low temperature stress in maize in northeastern China needs to be solved urgently. The chilling injury induced ZmRLK expression, and the expression level was proportional to the low temperature treatment time. RLK is a leucine-rich transmembrane region-like receptor kinase, which plays an important role in the fight against abiotic stress. The overexpression of ZmRLK can enhance plant tolerance to chilling injury. In this study, the immature embryos of maize inbred line HiII were selected as the acceptor material, and the cold-resistant gene RLK was transformed into the immature embryo by Agrobacterium tumefaciens-mediated transformation. The embryogenic callus was induced