黄叶地不容生物碱成分提取分离、结构修饰及活性评价-药物化学专业论文.docxVIP

贵州大学 贵州大学 2009 届硕士研究生学位论文 II II 摘 要 为了对千金藤属植物生物碱成分的全面了解及其药用机理的深入研究，本论 文对产自中国的千金藤属植物生物碱成分及活性研究进展进行了比较全面的综 述。 对黄叶地不容块根中的生物碱成分进行了系统的研究,运用药物化学研究领 域常见的多种分离材料和分离手段，结合现代波谱分析技术，从黄叶地不容块根 中分离鉴定了 15 个生物碱；其中有 3 个新的生物碱 (化合物 12、14、15)、4 个 化合物 (化合物 6、8、9 和 13) 为首次从该属植物中分离、5 个化合物 (化合物 3、4、5、7、10) 为首次从该种植物中分离得到；并对以上化合物的生物合成途 径进行了分析。 对黄叶地不容中的两个生物碱进行了初步结构修饰，并对部分提取物及单体 生物碱进行了生物活性筛选。通过对两个生物碱成分的初步结构修饰，取得一些 有意义的实验结果；活性筛选显示：样品 LF、化合物 (1、3) 对 K562 细胞有微 弱抑制活性；样品 LF、化合物 (1、2、3、4、6) 对 H2O2 诱导的 PC12 细胞损伤 有一定保护作用；样品 CT、LF 及化合物 (3、4、6) 对丁酰胆碱酯酶有微弱抑 制作用，化合物 (2、3、4、6) 对乙酰胆碱酯酶有极微弱抑制作用，CT、LF 及 化合物 1 对乙酰胆碱酯酶有微弱的抑制作用；样品 LF 对枯草芽孢杆菌、金黄色 葡萄球菌、藤黄微球菌均有抑制作用。 关键词：黄叶地不容；生物碱；光谱分析；生物活性 III III ABSTRACT In order to study the alkaloids constituents of Stephania plants, and to find out their medical mechanism and make full use of these natural medical resources. Research progress on the alkaloids constituents and their bioactivities from Chinese Stephaniagenus were reviewed in the article. This thesis systematically elaborated the alkaloids of Stephania viridiflavens H.S.Lo et M. Yang’s tubers. By a variety of chromatographies over various materials, fifteen alkaloids were isolated from S. viridiflavens’s tubers, including three new alkaloids (compounds 12, 14, 15). Their structures were mainly elucidated on the basis of spectroscopic methods. Four of them (compounds 6, 8, 9 and 13) were isolated from the Stephania genus for the first time, and five of them (compounds 3333, 4444, 5, 7, 10 and 11) were isolated from S.viridiflavens for the first time. The biosynthesis pathways of main alkaloids from S.viridiflavenswere analyzed. Two alkaloids were preliminary chemically modified. The extractives and some pure alkaloids from S. viridiflavens were tested on activities. Two of them were chemically modified and usefull results were found. Furthermore, the antibiosis, antitumor, antioxygen and antisenile dementia activities of the extractives and some pure compounds were tested. As resultes, sample LF and comp