甲基泼尼松龙对大鼠尺骨皮质骨骨量、微结构及力学性能的影响-代谢内分泌学专业论文.docxVIP

硕士学位论文 硕士学位论文 中文摘要 摘要 目的：研究甲基泼尼松龙对大鼠尺骨皮质骨量骨量、微结构及力 学性能的影响， 方法：20只3．5月龄雌-I生Sprague．Dawley大鼠，随机分为甲基泼尼 松龙干预组(3．5mg／kg／d)和对照组，每组10只毛干预后9周处死，处 死前测量体重及全身骨密度。剥离左侧尺骨行骨密度及三点弯曲压缩 力学实验。右侧前肢经肱骨肩关节离断后，行尺骨单轴疲劳，疲劳后 取尺骨中段行大块组织碱性品红染色包埋，行骨组织形态计量学测定 及微损伤定量分析。 结果：甲基泼尼松龙干预后，大鼠体重、全身骨密度和左侧尺骨 骨密度分别较对照组下降15％、6．4％和4．3％，尺骨内径周长和骨髓 腔面积较对照组分别增加23．3％和32％，外径周长较对照组下降3．1％ (PO．05)；尺骨皮质骨骨面积和截面总面积与对照组相比无统计学 差异。干预组大鼠的尺骨微损伤数量、微损伤数量密度、损伤表面长 度密度(2．33+0．71、1．20+0．39和1296+391)较对照组(4．16+0．31、 2．30士0．27和2592士387)分别增加43％、48％及50％愀0．05)，微损 伤平均长度无明显改变。干预组大鼠尺骨的最大力、最大挠度及弹性 模量20．7+0．8N、2．00士0．1 5mm及6．45+0．83MPa较对照组24．8士1．7N， 2．52士0．17mm及10．3土1．6MPa分别明显下降16．4％、37．5％和20．8％ (PO．05)。 结论：甲基泼尼松龙能显著诱导大鼠尺骨皮质骨骨量丢失、微结 构退变及力学性能的下降。 关键词糖皮质激素，骨密度，微结构，疲劳损伤，大鼠 硕士学位论文 硕士学位论文 英文摘要 ABSTRACT Objective To evaluate the effect of methylprednisolone on the ulna cortical bone bone mass，microarchitecture and mechanical performance in rats． Methods Twenty female Sprague-Dawley rats of three and half-month-old were randomly assigned to two groups：rats that were subcutaneously injected with methylprednisolone 3．5mg／kg per day (treatment group)，and rats that were subcutaneously inj ected with normal saline as vehicle(contr01)．Rats were sacrificed at 9 weeks after these treatments while body weight and total bone mineral density(BMD)were measured before they were killed．The left ulna was harvested for BMD measurement and the three point bending test．Single axial fatigue loading was performed on the right ulna,bone histomorphometry and microdamage analyses were performed on the middle ulna section． Results Body weight(一1 5％)，total bone BMD(一6．4％)and the ulnas BMD(-4．3％)in treatment group were significantly lower than those of the control rats(PO．05)．The ulna inner perimeter and marrow area in treatment group were increased by 23．3％and 32％respectively,while the outer perimeter were decreased 3．1％俨O．05)．No significant differen