免疫初乳与双歧杆菌复合微胶囊保护剂的筛选.PDFVIP

※生物工程 食品科学 2011, Vol. 32, No. 13 179 杨 柳，尤丽新，张英楠，陈海燕，马井喜 (吉林农业大学发展学院，吉林 长春 130600) 以双歧杆菌发酵免疫初乳，然后采用真空冷冻干燥技术研制成冻干粉，并以冻干粉为芯材、以肠溶材料 欧巴代为壁材，采用空气悬浮微胶囊化的方法制成免疫与微生态双活性肠溶制剂。加工过程中加入菌体保护剂，通 过单因素试验和正交试验确定微胶囊的保护剂的最佳配方为：海藻糖添加量为 6% ，水解酪蛋白添加量为6% ，乳 化剂 Span80 添加量为 3% 。得到的微胶囊中活菌数为6.0 ×108CFU/mL ，存活率可达 18.2%，比未添加保护剂的对 照组活菌存活率提高了 16.8%。 双歧杆菌；微胶囊；保护剂 Screening for Optimal Protectant for Compound Microcapsule of Immune Colostrum and Bifidobacteria YANG Liu ，YOU Li-xin ，ZHANG Ying-nan ，CHEN Hai-yan ，MA Jing-xi (Development College, Jilin Agricultural University, Changchun 130600, China) Abstract ：Immune colostrum was fermented by Bifidobacterium adolescentis and then subjected to vacuum freeze-drying to obtain freeze-dried powder. The prepared freeze-dried powder was used as the core material and CAP Opadry was used as the wall material to prepare an enteric-coated preparation with both immune and microbial activity by an air suspension microencapsulation method with the addition of protectant. The optimal protectant composition for improved viable Bifidobacterium adolescentis count was determined by single factor and orthogonal array design to be trehalose of 6%, hydrolyzed casein of 6%, and emulsifier Span80 of 3%. The viable bifidobacteria count in the obtained microcapsules was 6.0 × 108 CFU/mL and the survival rate of bifidobacteria was 18.2%, which exhibited an increase of 16.8% when compared to the control with the addition of protectant. Key words ：Bifidobacterium ；microcapsule ；protectant 中图分类号： TS201.3 文献标识码：A 文章编号：1002-6630(2011)13-0179-05 初乳是指雌性哺乳动物分娩后 7d 内所分泌的乳汁的 抑制肿瘤等一系列的生物功能[3-5] 。 统称。初乳与普通乳汁比较，其特殊性首先体现在化 免疫乳是指对哺乳动物( 主要是牛、羊等) 选择性地 学组成上：初