碰撞诱导裂解-电子转移解离CID-ETD质谱对糖基化多肽的分析.PDF

※基础研究 食品科学 2013, Vol.34, No.17 43 碰撞诱导裂解-电子转移解离(CID-ETD) 质谱对糖基化多肽的分析 1 1 2,* 1 刘光宪 ，管珊红，王 辉 ，冯健雄 (1.江西省农业科学院，江西 南昌 330200 ；2.南昌大学生物质转化教育部工程中心，江西 南昌 330047) 摘 要：采用碰撞诱导裂解- 电子转移解离液相串联质谱(LC LTQ-ESI CID/ETD MS-MS)分析胃蛋白酶酶解后的糖基 化卵清蛋白肽。结果表明：碰撞诱导裂解一级质谱(CID MS)可以对糖基化多肽进行初步识别和鉴定；碰撞诱导裂解 二级质谱(CID MS-MS)可以作为通过裂解多肽链实现部分糖基化肽位点的定位分析，但峰值较小，杂峰较多，形成 了部分中性丢失的新峰。电子转移解离二级质谱(ETD MS-MS) 比较适合于氨基酸序列长度为中等，带2个电荷的糖 基化修饰肽段的裂解，对于较大分子质量的糖基化修饰肽的ETD( 电子转移解离)裂解片段较少，判定方面存在一定 困难，因此，需要发展碰撞诱导裂解中性丢失三级质谱(CID-NL-MS3)技术对分子质量较大糖基化肽的糖基定位及 定性分析。 关键词：糖基化肽；卵清蛋白；碰撞诱导裂解；电子转移解离；质谱 Analysis of Glycated Peptide by CID-ETD Mass Spectrometry 1 1 2,* 1 LIU Guang-xian ，GUAN Shan-hong ，WANG Hui ，FENG Jian-xiong (1. Jiangxi Academy of Agricultural Sciences, Nanchang 330200, China ； 2. Engineering Research Center for Biomass Conversion, Nanchang University, Nanchang 330047, China) Abstract ：Identification of glycation sites in glycation protein plays an important role in the fields of food science and - medicine. In this work, liquid chromatography (LC) coupled with linear ion trap quadrupole electrospray ionization tandem mass spectrometry (LTQ-ESI CID/ETD MS-MS) was used to analyze peptides from glycated ovalbumin digested by pepsin. The results indicated that primary identification of glycation peptides was carried out through CID MS spectrometry and site identification through CID MS-MS fragment ions. However, new ion peaks from CID MS-MS analysis were very weak and complex with some various degrees of neutral