壳聚糖固定化木瓜蛋白酶提取牛蒡多糖的研究.PDF

226 2007, Vol. 28, No. 09 食品科学 ※工艺技术 高明侠，苗敬芝，曹泽虹，董玉玮，秦卫东，吕兆启 (徐州工程学院生物工程学院，江苏 徐州　 221008) ：以壳聚糖为载体、戊二醛为交联剂固定化木瓜蛋白酶，从牛蒡中提取多糖，考察固定化工艺的选择、 固定化酶提取牛蒡多糖的优化条件及固定化酶的稳定性。结果表明：壳聚糖固定化木瓜蛋白酶的最佳条件为：壳 聚糖浓度2.5%，加酶量0.3g/g 载体，时间6h，温度15℃，pH7.5，酶活力回收率38.98%。提取牛蒡多糖的最 佳条件为：固液比 1:20，pH6.5，温度60℃，时间8h，加酶量 1.8g/g 载体，多糖提取率11.04%。固定化酶重 复使用五次，酶活力仍保持50% 以上。 ：壳聚糖；固定化；木瓜蛋白酶；牛蒡多糖 Study on Extraction of Burdock Polysaccharide Using Immobilized Papain by Chitosan .. GAO Ming-xia，MIAO Jing-zhi，CAO Ze-hong，DONG Yu-wei，QIN Wei-dong，LU Zhao-qi (Bioengineering School, Xuzhou Institute of Technology, Xuzhou 221008, China) Abstract ：Chitosan was used as carrier and glutaraldehyde was used as cross-linking agent for the immobilization of papain. The immobilized conditions and stability of immobilized papain as well as its application in extraction of burdock polysaccha- ride were studied. The results showed that optimal conditions of immobilization of papain were 2.5% chitosan concentrations, 0.3 g papain/g carrier, 6 h times, 15 ℃ temperature, pH7.5. The recovery activity of immobilized papain was 38.98%. The optimal conditions of extraction of burdock polysaccharide were 1:20 ratios of solid to liquid (W/V), pH6.5, 60 ℃temperature, 8h hydrolyzed times, added 1.8g immobilized papain/g carrier. The extracting ratio of burdock polysaccharide was 11.04%. The more than 50% after repeated 5 times. activity of immobilized papain remained Key words：chitosan；immobilization；papain；burdock polysaccharide 中图分类号：O629.12 文献标识码：A 文章编号：1002-6630(2007)09-0226-04 壳聚糖(chitos