鸡和鹌鹑属间杂交种睾丸发育不良机制的初步探索 -遗传学专业论文.docxVIP

II II Abst r act The hybrid of chicken-quail is a typical successful instance in poultry in hybridization.But the hatching rate of it is low ,and the testis of it is dysplastic ,and the hybrid of it is kakogenic, so it can not be used in production.Testis is the main reproductive organs and spermatogenesis places of male animal,but also it is a workplace where some certain hormone synthesis and secretion , it plays a key role on physiological and developmental. The testicular growth and development is affected by several factors. AR, ER genes may play a important role in testis development. The testis development is a cell proliferation and apoptosis process, and BCL-2 and PCNA is related genes which can effect the cell development and apoptosis. This study investigate the developmental expression patterns of AR、ER、PCNA and Bcl-2 genes in testis, using the method of Real time PCR. Meanwhile the morphological changes of testis were investigated by histo1ogical method.The results are shown as follows: The morphological changes of testis in chicken、quail and the hybrid of chicken-quail Application Anatomy and histology method on chickens, quail and the hybrid of chicken-quai of a comparative study of testicular morphology. Analysis the different testis in the method of anatomy and histology. Results:(1)The color of hybrids testis is similar with the cheeses. The testis position and shape of chicken、quail and the hybrid of chicken-quail are different.It is smaller than chicken and quail.(2) The diameter of seminiferous tubules,the length of Seminiferous epithelium ,and the volume density of seminiferous tubules are smaller than that of chicken and quail.In addition,the number of spermatogenic cell and sertoli cell is lower. Development patterns of testicular AR、ER mRNA expression in cock、quail and the hybrid of chicken-quail. (1) The expression of AR mRNA was increased then down in cock and quail. But a tendency of step-wise increase was observed in expressio