马铃薯黄萎病病原菌营养亲和群生理小种交配型鉴定以及致病力.PDF

植物保护学报Journal of Plant Protection ，2018 ，45 （6）：1212-1219 DOI ：10.13802/j.cnki.zwbhxb.2018.2017118 马铃薯黄萎病病原菌营养亲和群、生理小种、 交配型鉴定以及致病力差异分析 赵晓军 张 键 张 贵 张园园 周洪友 赵 君* （内蒙古农业大学农学院，呼和浩特 010018 ） 摘要：为明确引起马铃薯黄萎病的大丽轮枝菌Verticillium dahliae 的遗传变异和致病力的差异，对 从内蒙古及其周边地区马铃薯发病株上分离得到的29 株大丽轮枝菌的营养亲和群、生理小种和交 配型进行了测定，利用伤根接种法进行致病力分化研究。结果表明，供试的29 株马铃薯大丽轮枝 菌菌株被划分为VCG2B 、VCG4B 和VCG4A 三个营养亲和群，其菌株数分别为11、2 和16株；供试 的29 株菌株均鉴定为单一的2 号生理小种和MAT 1-2-1 交配型。供试的29 株大丽轮枝菌菌株间致 病力存在一定的差异，其中NCP- 1 菌株的致病力最强，病情指数为83.33 ；而NWS-5 菌株的致病力 最弱，病情指数仅为10.85；不同营养亲和群菌株的平均致病力也存在显著差异，VCG4B 型菌株的 平均致病力最高，平均病情指数为67.18；其次为VCB2B 型菌株，平均病情指数为42.50 ，而VCG4A 型菌株致病力最弱，平均病情指数仅为20.54。 关键词：马铃薯黄萎病；营养亲和群；生理小种；交配型；致病力分化 Identification of the vegetative compatibility groups (VCGs), races, mating types, and pathogenicity differentiation of pathogenic bacteria of potato Verticillium wilt Zhao Xiaojun Zhang Jian Zhang Gui Zhang Yuanyuan Zhou Hongyou Zhao Jun* （Department of Agronomy, Inner Mongolia Agricultural University, Huhhot 010018, Inner Mongolia Autonomous Region, China ） Abstract: In order to clarify the genetic variation and pathogenicity differentiation in the pathogenic bacteria of potato Verticillium wilt, 29 strains were isolated from diseased samples which were collected from different potato fields in Inner Mongolia and their surrounding areas, and then the VCG types, physiological races, mating types and pathogenicity differentiation were measured and the pathogenici- ty of all tested strains was studied by using root-drenching method. The results showed that 29 strains could be divided into three VCGs, namely VCG2B ( 11 strains), VCG4B (two strains), and VCG4A ( 16 strains), which were identified as Race 2 and MAT1-2-1. There were signif