具有金属螯合活性的蚕豆蛋白酶解物的制备.pdf

6 10 Vol. 6 No. 10 2015 10 Journal of Food Safety and Quality Oct. , 2015 * 李雪芬, 丁 轲, 韩 涛 , 陈湘宁 (, 102206) 摘 要: 目的 , 方法 , , , 结果 3 , , 4 h , 88.22%, 220.70 U/mg, 0.03 U/mg结论 , , , 0.9250.968(P0.01) 关键词: ; ; ; ; Preparation of metal chelating peptides from broad bean protein by enzymatic hydrolysis * LI Xue-Fen, DING Ke, HAN Tao , CHEN Xiang-Ning (College of Food Science and Engineering, Beijing University of Agriculture, Beijing 102206, China) ABSTRACT: Objective To investigate the metal-chelating abilities of broad bean protein hydrolysates and the relationship between the metal-chelating abilities and the antioxidant activities of protein hydrolysates from broad bean. Methods Broad bean proteins were hydrolyzed by papain protease, neutral protease and alcalase, respectively. Then, the metal-chelating abilities and the antioxidant activities of the hydrolysates were evaluated. To evaluate comprehensive condition of hydrolysis and metal-chelating abilities, alcalase was employed as the suitable protease for studying the hydrolysis from broad bean. Degree of hydrolysis (DH) a