两亲性嵌段共聚物荧光标记方法.pdfVIP

两亲性嵌段共聚物荧光标记方法 夏自花, 刘意, 于丽萍, 俞安安, 杨帆. (上海理工大学材料科学系，上海 200237) Abstract： Rhodamine B (RhB) was used to decorate an amphipathic block polymers (β-CD-[P(AA-co-MMA)-b- PVP] ) in this study. First, after activated by 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, 4 rhodamine B was marked with hydroxyethyl methacrylate (HEMA) through ester exchange reaction. Second, the labeled amphipathic block polymers (β-CD-[P(AA-(HEMA-RhB)-MMA)-b-PVP] ) were 4 synthesized after polymerization reaction of double bones between RhB-HEMA and other reactants. Finally, the structure of product was measured by FT-IR spectra and fluorospectro photometer (FLUORO). The critical micelle concentration of RhB-labeled and unlabeled amphipathic block polymers were 4.96×10-3, 5.09×10-3 mg·L-1, respectively, indicating no change of their micellization behavior. In vivotissue distribution and whole-body fluorescent imaging were studied by vinpocetine (VP)-loaded polymeric micelles which were prepared through a solvent evaporation method. Compared to the result of in vivo tissue distribution and whole-body fluorescence imaging, a similar bio-distribution behavior of VP-loaded polymeric micelles was found. Those proved the successful fluorescence modification with a labeling yield of 4.13%. With in vivo fluorescence imaging technology, we established a fluorescence method for modification of amphipathic block polymers. 摘要： 结合活体荧光成像技术，为基于 β-环糊精的两亲性嵌段 4 臂聚合物（β-CD-[P （AA- co-MMA）-b-PVP] ）建立一种荧光标记方法。通过酯交换反应为罗丹明B 分子中引入含 4 有双键的甲基丙烯酸羟乙酯，然后通过双键聚合反应将罗丹明B 标记到两亲性嵌段共聚 物上。借助红外、荧光光谱对标记产物进行表征，并通过紫外分光光度法计算荧光标记 产率；制备长春西汀（VP）荧光载药胶束，进行动物组织分布实验和小动物活体荧光成 像实验，对比两个实验结果来验证荧光标记结果。荧光标记产率为4.13%；荧光标记前