氯喹在红内期灭活全虫疫苗抗红内期红外期中的作用及机制-微生物学专业毕业论文.docxVIP

第三军医大学硕士学位论文Chloroquine 第三军医大学硕士学位论文 Chloroquine enhance the protection efficacy of whole-·killed blood stage vaccine against malaria blood stage，but not the liver stage Abstract Malaria remains a fatal public health problem resulting in high morbidity and mortality in tropical and subtropical regions[2]，it is initiated by the bite of an infected anopheline female mosquito with the inoculation of sporozoites[1]．A high—efficiency vaccine is regarded as the most promising and cost-effective tool for malaria contr01．Although the scientists have made great efforts to develop subunit vaccines against malaria in the post fifty years，no high-efficiency subunit vaccine is licensed．The failure is greatly attributed to the only one or several antigens are involved in the subunit malaria vaccine designation， and high polymorphisms of the target antigens existing among the different strains of malaria parasites． In contrast，a whole-organism vaccine with maximal spectrum of antigens was appreciated recently．The whole—organism vaccines，such as irradiation。or genetically attenuated sporozoite and whole-killed blood-stage vaccine，have been demonstrated to induce the protective immunity in both human and mice．Of those，whole·killed blood。stage vaccine was prepared by repetitive freeze·thaw of the blood-stage infected RBCs，and immunized the mice along with alum．Our previously research have showed that immunization of whole．killed blood．stage vaccine could induce the protective immunity, but needed a high dose of pRBCs，which limits its wide application．Additionally,the protection efficacy needs to be greatly improved．A strong adjuvant has been demonstrated as a solution to this problem． Chloroquine，which was discovered in 1 93 4，was used as an antimalarial drug since 1 94714]．It shows its maximum efficiency when the parasites stays in RBCs while it is ineffective against sporozoites．Several studies reported that chloroquine can regulate host immune responses．However,whether it could in