黄瓜耐低氮胁迫生理及分子机制初步研究-园艺学；蔬菜学专业毕业论文.docxVIP

C0_n研rrSCoNTENTS C0_n研rrS CoNTENTS Abstract in Chlnese I Abstract in English ．．． ． ．． ．． ．．III 1．Introduction ．．1 1．1 Advantes in domestic and overseas ．1 1．1．1 Significance ofresearch onlownitrogentolerance ofplant ．． ．． ．．1 1．1．2 Physiological and molecular mechanisms of plant response to low nitrogen ．．1 1．1．3 NAC transcription factor gene family 4 1．1．4 Detection method ofplant nutrition condition ．．5 1．2 Aims and significance ofresearch ． ．．． ．．， ． ．， ． ．6 1．2．1 Aims ofresearch ．．6 1．2．2 Significance ofresearch ． ．．．．6 1．3 Experimental content and technical route ．6 1．3．1 Experimental main content ． ．． ．．． ，6 1．3．2 Experimental main technique course ．6 2．Materials and Methods 7 2．1 Auxin positively regulates nitrogen remobilization in cucumber leaf under low nitrogen tolerance during reproduvtive stage 7 2．1．1 Experimental materials． ． ． ．．．．． ． ．．． ．．．．． ．．7 2．1．2 Experimental methods ．．7 2．2 Cloning and functional analysis of low nitrogen tolerance related gene CsNAC036 in cucumber ．．． ． ．． ．．． ．．． ． ．10 2．2．1 Experimental materials ．． ．．． ． ．．．．， ． ．．10 2．2．2 Experimental methods ． ． ． ．．．．．．． ． ． 12 2．3 CsCHS is marker gene for low nitrogen tolerance in cucumber．．．．．．． ． ．．．16 2．3．1 Experimental materials ． ．． ．． ．．． ． ．． ．．．．． ．． ． ．16 2．3．2 Experimental methods ． ．．． ．．17 3．Results analysis ．．．，． ．． ． ．．，． ． ．．．．．． ．．．18 3，1 Auxin positively regulates nitrogen remobilization in cucumber leaf under low ni仃ogen tolerance during reproduvtive stage ．． 18 3．1．1 Nitrogen remobilization efficiency is of the key factors that affe