麦麸黄酮化合物的提取及抗氧化活性评价研究-食品加工与安全专业毕业论文.docxVIP

万方数据 万方数据 摘要 麦麸是我国主要的粮食加工副产物，黄酮是其主要活性成分。本论文以麦麸为 试验材料，研究麦麸黄酮提取方法、大孔树脂富集工艺，并对麦麸黄酮的体外抗氧 化活性进行了评价，为以后麦麸深精加工提供理论依据。 2本试验采用有机溶剂浸提法，通过选择合适的提取溶剂，对影响提取率的各个 单因素进行考察，筛选出影响显著的单因素，再利用响应面模型优化，建立麦麸黄 酮的最佳提取工艺。选用 6 种树脂对麦麸黄酮粗提物进行富集，通过静态吸附、解 吸试验筛选出最佳的树脂，然后通过对动态吸附、洗脱影响因素的考察，建立树脂 富集的最优工艺。在体外抗氧化试验中，以 Vc 为参照物，评价麦麸黄酮的还原力以 及对二苯代苦味酰自由基（DPPH?）、羟基自由基（OH?）、超氧阴离子自由基（O -?）、 总抗氧化力（ABTS+?）清除能力的大小。试验主要结论如下： 2 1、确定了对麦麸黄酮提取过程影响显著的三个因素：乙醇浓度、料液比、提取 时间；响应面分析法优化的最佳提取工艺条件：乙醇浓度 70%、提取时间 3h、料液 比 1:30，提取率达到 0.82%。 2、AB-8 型树脂对麦麸黄酮的富集效果最佳，具体条件：上样流速为 2BV/h， 上样浓度为 1.406mg/mL，洗脱剂为 60%的乙醇溶液，洗脱流速为 2BV/h。在最佳工 艺条件下，黄酮回收率达 61.95%，纯度为 22.6%，相比之前纯度提高了 3.7 倍。 23、通过体外抗氧化试验，发现提取得到的麦麸总黄酮抗氧化能力较强，且与浓 度存在量效关系。在 Vc 作参比的条件下，富集物对 DPPH?、O -?的清除率明显高于 麦麸粗提物；富集物和粗提物对 OH?清除效果相当；粗提物在还原力和对 ABTS+? 的清除能力方面优于富集物。 2 关键词：麦麸；黄酮；富集；体外抗氧化活性 I Abstract In China, wheat bran is the main grain residues. The extraction technology of wheat bran flavonoids, antioxidative capacity, purification method and character of flavonoids were mainly studied in this paper. This work provides a theoretical basis for the finishing raw wheat bran. Firstly, the experiment investigated single factors of the extraction to find out the important factors. Then the response surface model was built to optimize the optimum process conditions of extracting flavonoids from wheat bran. Secondly, crude extract were enriched by six kinds of resins. By static adsorption experiment, the optimum resin was selected. Through investigating the factors of dynamic adsorption and elution, the purification of wheat bran were obtained by optimum conditions. Finally, the oxidationresistant experiments were studied by different concentration of flavonoids from wheat bran 、 Vc, such as reducing power, scavenging activity on 21,1-dipheny-l-2-picrylhdrazyl(DPPH?) 、 superoxide radical(O -?) 、 and hydroxyl radical(OH?) 、 the total antioxidant capacity(ABTS+?). The main conclusions of this reseach are as follows: 2 1 、 Three factors had significant effects on the extrac