墨兰及其杂种的组织培养与快速繁殖.pdf

Guihaia 　18 (2):153—156 1998 5 　 ＊ 曾宋君　程式君　张京丽　赵逢畔　黄向力 (, 510520) 　　、 , 、, F 1 、、 、。, 1/2 MS +NAA 0.5 mg/L +10 % , ;、 MS +6-BA 0.5 mg/L +NAA 0.5 mg/L +0.5 %, ;、、0.5% G3 ; B5 +6-BA 2 ～3 mg/L + NAA 0.2 mg/L +0.5%;1/2 MS +NAA 2.0 mg/L + 10% +10 %+1.0 %; 2%, 3%。, ; 、、、 、 、 。 　;;;;　 A study on tissue culture and rapid propagation of Cy m bid i um sinense and its hybrids in vitro Zeng SongjunCheng Shijun Zhang JingliZhao Feng an Huang Xiangli (S ou th China Bo tan ica l Garden, Ch inese Academy of S cience, Guangzhou 510520) Abstract 　In this paper, the tissue culture and rapid propagation of em ryo, shoot, flower ud, leaf and root of Cy mbidium sinense and its hy rids were studied.the results are as follows:The suita le mediums are 1/2 MS +NAA 0 .5 mg/L +10 %coconut juice in em ryo sprouting and MS +6- BA 0.5 mg/L +NAA 0.5 mg/L +0.5 %active car on in culture of shoot and flower ud.The culture of leaf and root had not succeeded.G3 that mainly includes Plyponex No.1, Plyponex No.2, Bacto-peptone and active car on is the est medium in propagating of khizomei .The optimun medium of inducing udsform khizomei is B5 +6-BA 2 ～3 mg/L +NAA 0 .2 mg/L +0.5 %active car- on.Growth of seedling and induction of root are the fastest in 1/2 MS+NAA 2 .0 mg/L+10% a- nana juice +10 %applejuice.The optimun sucrose concentration of medium is 3%except the medium 　1997-08-11 　:, , 1965 , , , 。 ＊ 154 　　　 18 　　 in inducing root, w hich is 2 %.Hy rids have heterosis to its mother in khizomei propagation, ud and root induction, seedling grow th, disease resistance and so on. Key words 　Cy mbidium sinense;protocorm-li