水溶性茶绿色素的提取及性质研究.pdf

23 3 Vol. 23 No. 3 2003 9 hemistry and Industry of Forest Products Sept. 2003 杨晓萍, 郭大勇 ( 华中农业大学 园艺林学学院, 湖北武汉430070) YANG X P : , :1% NaOH 80 90 min ; pH 3. 0, 80 60 min, ; pH 2.2~ 5.0 , , , , : ; :Q949.758.4; TS202.3 :A : 0253- 2417(2003)03- 0069- 04 STUDY ON EXTRA TION AND HARA TERIZATION OF WATER-SOLUBLE TEA GREEN PIGMENT YANG Xiao-ping, GUO Da-yong ( H uaz hong A g ricul ture Un iv ers ity of H or tic ul ture and For estry , Wuhan 430070, Chi na) Abstract: Water-soluble tea green pigment( TGP) were extracted from low grade tea leaf and waste tea etc. Physico- chemical propertiesand processes on saponification and copper substitution reaction were investigated.Results show ed that optimum condition of saponification is sodium hydroxide 1% for 90 min,temperature of water bath 80 .At pH value 3. 0, temperature of water bath 80 for 60 min, a high proportion sodium copper chlorophyllin w as achieved.The pH value in the range of 2.2- 5. 0 had no effect on the pigment .Heat and most of metal ions exerted insignificant effect, while ascorbic acid had protective effect on the tone of pigment. Key words:tea green pigment; saponification ( tea green pigment, TGP) , , , , , , , [ 1] , , , [2] :2002- 10- 10 :( 1971- ) , , , , 70 23 , , , , , 1 1. 1 1.2 1.2. 1 水溶性茶绿色素制备工艺流程[ 2~ 3] 1.2.2 水溶性茶绿色素稳定性的研究 0. 1% , , , 405 nm 2 2. 1 2. 1. 1 叶绿素皂化最优条件 90% , NaOH 41 , 1, , 652 nm , 1 ,