泥鳅胚胎玻璃化液超低温冷冻保存研究.pdf

26 3 Vol. 26, No. 3 2002 6 JOURNAL OF FISHERIES OF CHINA June, 2002 : 1000- 0615( 2002) 03- 0213- 06 1, 2 1 1 1 1 章龙珍 , 鲁大椿 , 柳 凌 , 郭 峰 , 张洁明 ( 1. , 434000; 2. , 200090) : ( ) 10# , ( - 196 e ) 17h , 3 e , B1B2B3 26.7% , 15.0% , 3. 0% 50h , : ; ; ; : S917 :A Cryopreservation of loach, Misgurnus anguillicaudatus, embryos by vitrification 1, 2 1 1 1 1 ZHANG Long-zhen , LU Da-chun , LIU Ling , GUO Feng , ZHANG Jie-ming ( 1. Yangtze R iver Fisheries Research Institute, Chinese Academy of Fisheries Science , Jingz hou 434000, China; 2. East China Sea Fisheries Research Institute, Chinese A cademy of Fisheries Science, Shanghai 200090, China Abstract: Use of a vitrified solution ( composition 10) as a cryoprotectant for the cryopreservation of loach embryos is studied in this paper. Using this vitrified solution, embryos were preserved at a temperature of - 196 e for 17 hours. Afterward, the preserved embryos were thawed using thawing solution in warm water ( 3 e ) , and revival embryos were successfully obtained. During thawing, we compared 3 kinds of thawing solution compositions ( B1, B2 and B3) , and the survival rates of the preserved embryos after thawing in B1, B2 and B3 thawing solutionswere 26. 7% , 15. 0% and 3. 0% respectively. After thaw ing, it took 50 hours for the development of revival embryos from the interval of blastopore closure to the interval of caudal fi