抗生素对山茶愈伤组织诱导和生长的影响.pdfVIP

林业科学研究 2005, 18(2) : 183~ 186 Forest Research : 10012 1498(2005) 022 01832 04 范正琪, 李纪元, 田 敏 ( 中国林业科学研究院亚热带林业研究所, 浙江 富阳 311400) :研究了卡那霉素、潮霉素对浙江红山茶、大花红花油茶 伤组织的生长及对攸县油茶幼苗叶片诱导 伤的影 - 1 - 1 响, 确定作为选择压力的卡那霉素质量浓度为50~ 60 mg# L , 潮霉素为25~ 30 mg#L 。在 3 种抑菌性抗生素中, 羧苄青霉素促进 伤组织诱导, 对 伤组织的生长没有影响, 头孢霉素则有抑制作用, 而羟氨苄青霉素轻微抑制 伤组织的生长, 但对 伤组织的诱导影响不大。考虑到抑菌效果, 适于在转基因研究中使用的羧苄青霉素的抑菌质 量浓度为250 mg#L- 1 , 羟氨苄青霉素为125 mg# L- 1 。 :抗生素; 山茶; 伤组织 + :S72213 7 :A Effect of Antibiotics on Induction and Growth of Camellia Callus FAN Zheng2qi, LI Ji2yuan, TIAN Min (Researc Institute of Subtropical Forestry, CAF, Fuyang 311400, Z ejiang , C ina) Abstr act :T e effect oft e supplement ofantibiotics on induction and growt of camellia callus was studied. Kannamycin and Hy2 gromycin were more sensitive to growt of callus of C. chekiangoleosa t an t at of C. magniflora . Growt of callus would be lim2 - 1 - 1 ited by Kannamycin at 50~ 60 mg#L and Hygromycin at 25~ 30 mg#L . T e leaves of C. grjisii seeding could not induced callus w en Kannamycin and Hygromycin arrived t e same concentration. Carbenicillin could accelerate induction ofcallus at cer2 tain degree but didnot affect t egrowt of callus. Amoxicillin in ibited slig tly t e growt of callus. Cefotaximes reduced bot t e ratio. According to t e effect of in ibiting Agrobacterium tumefaciens, concertration ofCarbenicillin and Amoxicillin were 250 mg - 1 - 1 #L and 125 mg#L respectively in t e study of transformation. Key wor ds: antibiotics; Camellia ;