川陈皮素Nobiletin-食品科学系.DOC

屏東科技大學食品科學系碩士班 研究生專題報告書面摘要 題目： 柑橘果皮中川陳皮素及其代謝產物之抗發炎活性 指導教授：黃卓治 博士 Anti-inflammatory activity of nobiletin and its 報告學生：王俊堯 metabolites of citrus peel 報告日期：2009/12/15 摘要 多甲氧基類黃酮發現在柑橘屬中，特別是在甜橘及柑橘的果皮中。最常被用來研究生理活性的是川陳皮素(Nobiletin)及橘皮素(Tangeretin)這兩種成分。在許多文獻指出川陳皮素具有許多生理活性，包括抗發炎、抗癌症及抗動脈粥狀硬化等，還有改善消化不良、支氣管和氣喘等症狀。在抗發炎部分，川陳皮素可抑制 nuclear factor κB (NF-κB) 轉錄的活性、nitric oxide (NO)及 prostaglandin E2 (PGE2)的產量及inducible nitric oxide synthase (iNOS)和Cyclooxygenase-2 (COX-2)蛋白的表現。另有文獻指出，川陳皮素在老鼠的體內會經由去甲基化途徑進行去甲基化，且在尿液中發現3’-demethylnobiletin、4’-demethylnobiletin及 3’,4’-demethylnobiletin。利用以上化合物作用於細胞後，發現川陳皮素代謝產物的消炎活性比川陳皮素強，特別是4’-demethylnobiletin及3’, 4’-demethylnobiletin可明顯的抑制iNOS和COX - 2基因的表現進而發揮其抗發炎和抗癌功能。在其他研究的報告中，川陳皮素經由Aspergillus niger的生物轉化後產生4 - demethylnobiletin生理活性。我們將研究Aspergillus niger轉化川陳皮素代謝產物的抗炎活性。 關鍵字：川陳皮素、多甲氧基類黃酮、抗發炎 Abstrac Polymethoxyflavones are found exclusively in the Citrus genus, particularly in the peel of sweet oranges (Citrus sinensis) and mandarin oranges (Citrus reticulate). Out of known polymethoxyflavones, nobiletin and tangeretin are the two most studied for their biological activities. There are many research reports about nobiletin, mainly about their biological activities, including anti-inflammatory, anti-carcinogenic, anti-atherogenic properties and treat indigestion, improve bronchial, asthmatic conditions. In anti-inflammatory part, nobiletin suppressed nuclear factor κB (NF-κB) transcriptional activation, nitric oxide (NO) and prostaglandin E2 (PGE2) production, and inducible nitric oxide synthase (iNOS) and Cyclooxygenase-2 (COX-2) protein expression. The biotransformation study of nobiletin has shown that it undergoes mono-demethylation (3’- and 4’-demethylnobiletin) and di-demethylation (3’,4’-didemethylnobiletin) metabolic pathway. Biological screening of nobiletin and its metabolites has revealed that the met