Ⅰ突变体K3A-HWTX-Ⅰ的化学合成与生理活性分析-湖南师范大学自然.PDFVIP

2 2 Vol. 2 No. 2 1998 6 Life Science Research Jun. 1998 - K3A-HWTX- X 王贤纯　梁宋平　　　　　　　　罗泽民　　 ( , ,410081) ( , ,410128) 采用固相F oc 法在自制自动蛋白质化学工作站上合成了 第3 位赖氨酸( 3) 被丙氨酸( ) 取代的虎纹捕鸟蛛毒素- Ⅰ的突变体 3 - - K A K A HWTX Ⅰ. 合成的突变体用Ed an 降解和电喷雾质谱进行鉴定. K3A-HWTX- Ⅰ经谷 胱甘肽系统进行氧化复性后通过离子交换和反相HPLC 纯化. 活性分析结果表 明, 3 被 取代后 - Ⅰ的生物活性下降了80%,提示 3 与 - Ⅰ K A HWTX K HWTX 的生物活性有一定的相关性. 蜘蛛, 虎纹捕鸟蛛毒素- Ⅰ, 突变体, 固相多肽合成 Q516,Q966,R99 Chemical Synthesis and Physiological Activity Analysis of K3A-HWTX-:A Mutant of Huwentoxin- Wang Xianchun Liang Songping ( Depart ent of Biology, Hunan Nor al U niversity,Changsha, 410081, PRC) Luo Ze in ( Depart ent of Biological Technology,Hunan Agricultural University,Changsha,410128,PRC) 3 - -, - Abst act K A HWTX a utant of the neurotoxic peptide Huwentoxin I in which the 3 , residue Lys is replaced by Ala was synthesized by solid phaseche ical ethod on our au- to aticworking station for protein che istry.The synthetic peptide was identified by Ed- an degradation and ESI( Electrospray ionization) - ass spectru . K3A-HWTX-was - renatured in oxidative buffer containing glutathione and purified by ion exchange and re- . 3 versed phase HPLC Physiological experi ent indicated that the substitution of Ly