甘薯叶过氧化物酶的分离纯化及其部分性质研究.PDF

※生物工程 食品科学 2010, Vol. 31, No. 07 223 付伟丽，唐靓婷，王 松，朱 鸿，郜赵伟，唐云明* (西南大学生命科学学院，重庆市甘薯工程研究中心，三峡库区生态环境教育部重点实验室，重庆 400715) ：经硫酸铵分级沉淀、DEAE-Sepharose 离子交换层析、Sephacryl S-200 凝胶过滤层析，从甘薯叶中得到过 氧化物酶(POD) 电泳纯制品。该酶比活力为91923.14U/mg ，纯化倍数为255.69 ，回收率为1.59%。该酶分子量约 为35kD ，最适pH 值为5.6 ，最适温度为60 ℃。该酶在20 ～50 ℃、pH4 ～8 内稳定。以不同浓度H2 O2 为底物在 pH7.2 和25 ℃下测得该酶K m 值为0.291mol/L 。低浓度草酸、尿素、Li+ 、Na+ 、K+ 、Mg2+ 等对该酶有激活作用； SDS 、KSCN 、抗坏血酸(AsA) 、Mn 2+ 等对该酶有抑制作用；甲醇、乙醇、乙二醇、异丙醇对POD 均有一定 抑制作用，其抑制作用强弱顺序为异丙醇＞乙醇＞甲醇＞乙二醇。实验表明，该酶稳定性较强。 ：甘薯叶；过氧化物酶；分离纯化；性质 Isolation, Purification and Enzymological Properties of Peroxidase from Sweet Potato (Ipomoea batatas Lam.) Leaves FU Wei-li ，TANG Liang-ting ，WANG Song ，ZHU Hong ，GAO Zhao-wei ，TANG Yun-ming * (Key Laboratory of Eco-environments in Three Gorges Reservoir Region, Ministry of Education, Chongqing Sweetpotato Engineering Research Center, School of Life Science, Southwest University, Chongqing 400715, China) Abstract：In order to obtain purified peroxidase from sweet potato (Ipomoea batatas Lam.) leaves for the study of its enzymatic properties, ammonium sulfate precipitation, ion exchange chromatography on DEAE-Sepharose column and Sephacryl S-200 gel filtration were used to purify peroxidase (POD) from sweet potato leaves. The specific activity of purified POD was 91923.14 U/mg, which exhibited a purification fold of 255.69 and a recovery rate of 1.59%. The molecular weight of this enzyme was 35 kD, and the optimal pH and temperature were 5.6 and 60 ℃, respectively. The peroxidase was stable at 20－50 ℃ and pH 4 －8. The apparent Km of this enzyme using H2O2 as substrate at different conditions was 0.291 mol/L at 25 ℃ and pH 7.2. + + + 2+ 2+ It