改进的三羟基吲哚法测定肾上腺素.PDF

27 5 　　　　　 () Vol. 27№ . 5 2000 9 Journal of Zhejiang University( Sciences Edition) Sept. 2000 : 1008- 9497(2000)05- 0530- 04 1 2 3 童裳伦 ,吕国才 ,许志良 ( 1., 310028; 2., 310003; 3., 310006) 　: 本文利用改进的三羟基吲哚法测定肾上腺素. 实验表 ,在 = 8. 7的硼砂缓冲溶液中,肾上 pH 腺素用 K Fe(CN) 氧化后,加入巯基乙醇溶液,能稳定吲哚衍生物,从而可以在碱性条件下测定肾上腺 3 6 素,同时在该条件下多巴、多巴胺不能形成发荧光的产物,不干扰它的测定,由此提出了 一种灵敏、选择 性测定肾上腺素的分析方法. 　　: 三羟基吲哚法;肾上腺素;荧光光度法 + : T 460. 7 2　　　: A 1 2 3 TONG Chang-lun , LU Guo-cai , XU Zhi-liang ( 1. Department of Environmental Science, Zhej iang University , Hangzhou 310028, China; 2. The First Hosp ital of Zhej iang University , Hangzhou 310003, China; 3. Traditional Medical Hosp ital of Zhej iang , Hangzhou 310006, China) Determination of epinephrine by an improved modification of the trihydroxyindole method Journal of. Zhejiang University( Sciences Edition) , 2000,27( 5): 530～ 533 Abstract An improved modification of the trihydroxyindole method was used to determine epinephrine: in this paper. Experiments indicate that the fluorescent lutines can be stablized by the mercaptoethanol ( = 8. 7) . . in the borax buffer pH So it is possible to determine epinephrine in the alkaline medium Under the same conditions, dopa, dopamine can not form fluorescent products, therefore, they didn t interfere with the estimation of epinephrine From this a simple and selective method for the determination of. , epinephrine was put forward. Key wo