改良的DPPH与ABTS自由基法评价不同葡萄籽油抗氧化能力梁红敏.doc

收稿日期：2017-04-13作者简介：姓名梁红敏 收稿日期：2017-04-13 作者简介：姓名梁红敏，性别女，1987年出生，工程师，生物活性成分与功能性食品 通讯作者：姓名胡文效，性别男，1964年出生，研究员，生物化工专业生物分离 梁红敏 任继波 李彦奎 高德艳 胡文效* （山东省葡萄研究院，山东济南 250100） 摘 要 本研究采用改良的1,1-二苯基-2-三硝基苯肼（1,1-diphenyl-2-picryhydrazyl，DPPH）法和2,2’-联氮双-（3-乙基苯并噻唑林-6-磺酸）二胺盐（2,2’-azinobis（3-ethylbenzothi azoline-6-sulfonic acid）ammonium salt，ABTS）法测定不同方法提取葡萄籽油极性部分、非极性部分及全油的抗氧化能力，测定不同葡萄籽油多酚含量，研究多酚与葡萄籽油极性部分抗氧化能力的相关性。热榨毛油与溶剂法毛油在经过精炼后多酚含量和抗氧化能力显著降低；在溶剂法精炼油、热榨法精炼油、低温压榨油、超临界CO2萃取葡萄籽油中，低温压榨油极性部分清除DPPH与ABTS自由基的能力最强，低温压榨油的多酚含量最高，超临界CO2萃取葡萄籽油非极性部分与低温压榨油全油的DPPH与ABTS自由基清除能力最强；各不同葡萄籽油总酚含量与极性部分DPPH和ABTS自由基的清除能力具有显著的正相关性。 关键词 1,1-二苯基-2-三硝基苯肼（DPPH） 2,2’-联氮双-（3-乙基苯并噻唑林-6-磺酸）二胺盐（ABTS） 抗氧化能力 葡萄籽油 中图分类号：TS225.1 文献标识码：A 文章编号：1003-0174（ ） Modified DPPH and ABTS Assays to Assess the Antioxidant Capacity of Different Grape Seed Oils Liang Hongmin Ren Jibo Li Yankui Gao Deyan Hu Wenxiao* (Shandong Academy of Grape, Jinan 250100, China) Abstract This research dwells on two widely used spectrophotometric methods, 2,2-diphenyl-1-picrylhydrazyl (DPPH)and 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS) assays to assess the antioxidant capacity of grape seed oils extracted by different methods. The polyphenols contents in grape seed oils and the correlation of polyphenols and grape seed oils polarity part were determinated. Polyphenol content and antioxidant capacity of hot pressing oil and solvent oil were significantly reduced after refining. Among the solvent refined oil, hot press refined oil, cold pressing oil, supercritical CO2 extraction grape seed oil, the ability of cold pressing oil polarity part to remove DPPH and ABTS radical was stronger and its polyphenols content was the highest. the ability of supercritical CO2 extraction of grape seed oil nonpolar parts and cold pressed oil to remove DPPH and ABTS radical were stronger than others. Each grape seed oil, Total phenol content and the ability of to remove DPPH and ABTS radical of different grape seed oil