慢性间歇性低氧降低急性缺氧对大鼠PASMCs膜上K,v的抑制-生理学专业毕业论文.docxVIP

硕L学位论文 硕L学位论文 慢性间歇性低氧降低急性缺氧对大鼠PASMC膜上Kv的抑制 慢性间歇性低氧降低急性缺氧对大鼠 PASMCs膜上Kv的抑制 研究生：唐碧 导师：唐明教授 ABSTRACT Male SD rats were divided into two groups：control normoxic group and chronic intermittent hypoxic group．Using whole cell patch-clamp technique，voltage—gated potassium channel currents(1Kv)were recorded in freshly isolated pulmonary vascular smooth muscle cells(PASMCs)of rat with acute enzymatic digestion method．The effects ofacute hypoxia on IKv of PASMCs from chronic intermittent hypoxic group were investigated to determine the ionic mechanisms of the hypoxic acclimatization at the level of channels．The results showed：(1)In control normoxic group，after acute hypoxia perfusion，the resting memberane potential (Em) of PASMCs was depolarized significantly from(一47．24-2．6)mY to(一26．74-1．2)mY and peak current percent inhibition was up to(60．8±3．3)％at+60mV．and IKv of PASMCs was decreased significantly from(153．44-9．5)pA／pF to(60．14-10．6)pA／pF at +60mV，and current—voltage relationship curve shifted to the right．(2)In 堡．!兰竺堡兰 堡．!兰竺堡兰 丝堡塑鳖丝堡塑堂堡墨堡坠墼翌查塾坠!竺兰堕：!：坠!竺塑型 chronic intermittent hypoxic group，IKv of PASMCs was decreased significantly by exposure to intermittent hypoxia in a time dependent manner,IKv inhibition appeared to start at 10d and continued to 30d(the longest time tested)of hypoxia，and current-voltage relationship Culwe shifted to the fight in a time—dependent manner．(3)Compared with control normoxic group，percent IKv inhibition by acute hypoxia was significantly attenuated in chronic intermittent hypoxic group and this inhibition effect declined with time exposure to hypoxia．The results suggest that Kv current inhibition was significantly attenuated by chronic intermittent hypoxia，and this effect may be a critical mechanism of the body hypoxic acclimatization． Key words：pulmonary artery；voltage gated potassium channel；patch clamp；vascular smooth muscle ceils；chronic intermittent hypoxia；acute hypoxia． 颂L学位论文 颂L学位论文 慢性间歇性低氧降低急性缺氧对大鼠PASMC膜