米邦塔仙人掌多糖提取鉴定对及缺血性脑损伤的保护作用分析-药理学专业毕业论文.docxVIP

第Ⅲ部分 米邦塔仙人掌多糖对小鼠/大鼠脑缺血再灌注损伤的保 护作用及作用机制研究 （一） 米邦塔仙人掌多糖对小鼠脑缺血再灌注损伤的保护作用及机 制探讨 一．材料与方法 ·················································································48 二．结果 ·····························································································56 三．讨论 ·····························································································64 四．参考文献 ·····················································································66 （二） 米邦塔仙人掌多糖对大鼠脑缺血复灌损伤的保护作用机制探 讨 一．材料与方法 ·················································································68 二．结果 ·····························································································72 三．讨论 ·····························································································73 四．参考文献 ·····················································································74 本研究工作总结及创新点 ···································································77 综述 活性多糖在预防与治疗神经退行性疾病中的应用·························79 附录 研究生期间工作小结 ········································································93 致谢 ········································································································99 华 华 中 科 技 大 学 博 士 学 位 论 文 PAGE 10 PAGE 10 中英文缩略词表 middle cerebral artery occlusion MCAO 大脑中动脉阻塞 common carotid artery CCA 颈总动脉 permanent occlusion of the bilateral CCA 2VO 双侧颈总动脉永久性结扎 2’,7’-dichlorodihydrofluorescin diacetate DHCF-DA 2,7-二氯氢化荧光素 fura 2 acetoxylmethyl ester Fura 2/AM fura 2 乙酸甲酯衍生物 2,3,5-triphenylttrazolium TTC 2,3,5-三苯基氯化四氮唑 glumatic Glu 谷氨酸 opuntia milpa alta polysaccharides MAPs 米邦塔仙人掌多糖 gas chromatography-mass spectrometer GC-MS 气相色谱-质谱联用 hematoxylin-eosin HE 苏木素—伊红 gel permeation chromatography GPC 凝胶渗透色谱 brain derived neurotrophic factor BDNF 脑源性神经生长因子 high performance liquid chromatography intracellular calcium concentrations HPLC [Ca2+]i 高效液相色谱 细胞内钙离子浓度 lactate dehydrogenase LDH 乳酸脱氢酶 reactive oxygen species ROS 活性氧 o-phthalaldehyde