抗癌抗疟疾中草药化学成分的研究-物理化学专业毕业论文.docxVIP

摘 摘 要 白英(Solanum Lyratum Thunb．)，系茄科植物，现代医学研究表明白英具 有清热解毒、抗癌等作用，传统中医常用白英来治疗癌症，它对子宫颈癌、肺癌、 声带癌等有明显疗效，在临床上具有广泛的应用。 本文采用系统溶剂提取法对白英化学成分进行研究，依次用氯仿、乙酸乙酯 和乙醇进行冷浸提取，每种溶剂冷浸三次，每次7天。对提取得至U的浸膏采用大 孔树脂、硅胶柱层析和制备薄层层析进行分离纯化，乙酸乙酯部位分得化合物单 体5个，乙醇部位分得1个，经波谱(IR、MS、NMR)分析鉴定，对比标准图 谱和已经报道了的光谱数据，解析出其中4种化合物的结构，它们分别是薯蓣皂 甙元、槲皮素、柚皮素和白藜芦醇。其中槲皮素、柚皮素、白藜芦醇是首次从白 英中分离得到。 本文还利用水蒸气蒸馏法对白英挥发油成分进行了提取研究，收率O．09％， 经GC--MS分离鉴定，共得到10种主要成分，鉴定了其中9种的结构，它们分 别是水杨酸甲酯、1．(6．甲氧基-2一萘基)乙酮、十六酸甲酯、2，2，6一三甲基一1一【(1E)一3- 甲基一1。3-丁二烯基卜5一次甲基一7一氧杂二环【4，1．01庚烷、1,4一二甲基一8一(1一甲基亚乙 基)，2，3，4，5，7，8一六氢．6．甘菊酮、6，10，14一三甲基．2．十五烷酮、异植醇、9，12一十八二 烯酸和十六酸。其中前8种化合物系首次从自英中分离得到。 另外，本文对河南产青蒿中青蒿素进行了研究，对石油醚提取青蒿素的工艺 进行了优化，得出较佳的提取工艺条件，证实青蒿叶中青蒿素的含量较高。采用 薄层层析和柱层析的方法对青蒿的石油醚提取液进行分离纯化，结果表明此方法 简便、有效。 关键词：自英化学成分柱层析挥发油青蒿素 AbstractSolanum Abstract Solanum Lyratum Thunb．(solanaceae)，a herb ofSolanum，is used in Chinese medicine prescription widely．Modem pharmacology researches show that it is helpful to clear away heat and toxic material，its another function iS anti—cancer．In China． Solanaceae has been used as a remedy for various cancers，such as cervical carcinoma, pulmonary carcinoma，laryngo carcinoma．Solanaceae has extensive clinical applications，too． The chemical constituents ofsolanaceae were studied by systematic solvent extraction．The chemical constituents were extracted three times by chloroform，ethyl acetate and ethanol at room temperature in turn，every time takes 7 days．The extracts were isolated by macroporous resin，silica—gel and preparative TLC，As a resulL 6 compounds were isolated and 4 compounds were identified．The structures wer9 elucidated by IR，MS，1HNMR,13CNMR and were compared with standard spectral data or the know compounds reported in the literatures．Those structures are proposed as following：diosgenin，quercetin，naringenin and resveratr01．Among them，quercetin， naringenin and resveratrol were isolated from Solanaceae for the first time． In this dissertation．the volatile oil was extracted wit}l steam distillation．The extraction yield Was around O．09