蝴蝶兰胚性愈伤组织诱导的氧化还原动态-植物科学学报.PDF

武汉植物学研究 2010, 28(6): 737~743 Journal of Wuhan Botanical Research DOI: 10.3724/SP.J.1142.2010.60737 蝴蝶兰胚性愈伤组织诱导的氧化还原动态 刘福平, 陈丽璇 (, , 361006) (Phalaenopsis spp.), 11~18 d , 25~32 d 0~11 d, OH , O . 2 , H O , , , 2 2 , SOD , POD CAT , DPPH H O , SOD 2 2 , SOD , , SODO . H O 2 2 2 ; ; ; ; 中图分类号: Q943.1 文献标识码: A 文章编号: 1000-470X(2010)06-0737-07 Redox Dynamics During Embryogenic Callus Induction of Phalaenopsis spp. LIU Fu-Ping, CHEN Li-Xuan (Fujian Institute of Subtropical Botany , Fujian Key Laboratory of Physiology and Biochemistry for Subtropical Plants , Xiamen 361006, China) Abstract: Embryogenic callus was induced from protocorm-like body (PLB) dice of Phalaenopsis spp. Embryonic cells occurred on the 11th- 18th day of culture, and young globular-shaped embryoids appeared on the 25th-32th day. For the first 11 days, the relative content of  OH in cultures undulated slightly, O . production rate increased continuously and achieved its highest 2 value, and H O content increased significantly , meanwhile total antioxidative capability 2 2 remained low. The results suggested that oxidative stress on explants was relativel