铝对甘蔗初生根的胁迫及钙、硼、镁的缓解效应-植物营养学专业毕业论文.docxVIP

广西文学祯士学位论文铝对嚣蔗耪生根的胁逵及钙、熏、镁的缓瓣效应 广西文学祯士学位论文 铝对嚣蔗耪生根的胁逵及钙、熏、镁的缓瓣效应 攘要 镪的毒害被认为是酸性土壤上作物产量形成的主要障碍因子。镪毒 害的最初作用位点在根尖。本文通i毯营养液培养的方法对八个甘蔗品种 进行了耐铝性比较，并以桂糖15(GTl5)为材料研究了锚对初生根生长 的胁造及钙、硼、镁的缓解效应。研究发现，供试的八个甘蔗品种对铝 胁遮的耐·陛存在差异，TT24、GTl5分别是较耐锅和较不耐锅的两个品釉。 ， f铝对苗蔗(GTl5)初生根豹胁逵衣彩态上表现为：抑制根的伸长(20 t 肚mol·L。A1处理24小时即可明显抑制根的伸长，抑制率达50％以上)、 导数根尖弯酋、减少校尖的赣滚量；在生理上表现为诱导掇尖遂氧纯物 酶(POD)、超氯物岐化酶(SOD)活性的升高。但是铝的胁迫可以通过 增翻锈豹浓度寒缓解。当处理滚审锈鹃滚瘦爱1．0 mmol·L。时舔霹缓解 20 I，tmol·L。的铝对根伸长的抑制、减少铝在根尖的积累、抑制由于铝的 黪逑两导致的POD活整的提高。覆撩、镁(Mg 1．0 mmol·L。不簸起 到缓解铝对根胁迫的效应溉外，本文还对铝胁遮后GTl5根系的分泌物 ／ 进纷了研究。结果显示，锚黪逵裴诱导摄系分泌较多酶溪磊酸。 』 ／、 ≮ ／ 关键词：营蔗}纫生投}莲囊i萃p辩铝性熹每绔缓勰效鹿；鸯觐酸j 飞、 ( ￡耍查璺壅主兰堡燕兰—— ￡耍查璺壅主兰堡燕兰—— —— ALUMINUM STRESS ON INITIAL ROOTS OF SUGARCANE AND THE ALLEVIATING EFFECT BY Ca、B、Mg ABS善戳警C零 Phytotoxicity of aluminum(A1)is widely considered tO be a serious agricultnral problem limiting crop productivity in acidic soils。The primary site ofAl toxicity locates in root apices．In this study,the ability of AI—tolerance of eight sugarcane cultivars was identified by root elongation measuring and hematoxylin staining，and the effect of At on root growth ofGTl5 as well as some alleviating effect by applying Ca、B、Mg were deeply investigated．The results showed that different sugarcane cultivars differed in tolerance to AI stress，when TT24 and GTl5 were AI—tolerant and A1一sensitive cultivars respectively，In the presence of20 gmol·U1 A1，elongation ofinitial roots ofGTl5 was severely inhibited． The stress of A1 also resulted in curvature of root tips，and decreased the secretion of mucilage．In addition，AI stress pmmoted the activity ofPOD and SOD in root tip(6、12、 24酗．A1 was mainly accumulated in 0-10mm-segment of root tips．This region plus epidermis of the root tips were intensively stained by hematoxylin．But the stress induced by AI could be alleviated effectively by Ca．Under黼S circumstance，elongation of initial rootsWasonly repressed slightly,whereasthealleviating effectofBandMg(1．0mmol·L。) was not significant．The organic aci(1s seereted by roots