螺内酯、依那普利及其联用对梗阻性肾病大鼠肾间质纤维化的防治作用-内科学(肾病)专业毕业论文.docxVIP

硕士研究生论文 硕士研究生论文 中文摘要 (Po．05)，各药物干预组之间的比较无统计学差异(P0．05)，以依 那普利组表达最弱。③相关分析：各大鼠肾小管间质TGF．B l表达与 FN的表达呈显著正相关(r--0．893，P0．05)，与Masson染色纤维化 面积呈显著正相关(F0．787，P0．05)；各大鼠肾小管间质FN的表 达与Masson染色纤维化面积呈显著正相关(r=O．805，P0．05)。(3) WesternBlot显示，UUO术后14天，假手术组Col I蛋白的表达很少， UUO及各药物干预组Col I蛋白表达明显增高(po．05)；与UUO组 比较，各药物干预组Col I蛋白表达明显下调，以两药联用组表达最 弱(4)血生化指标：血钾浓度，UUO组及各药物干预与假手术组之间 比较，差异无统计学意义(PO．05)，而两药联用与UUO组相比较， 血钾浓度明显升高(PO．05)。血钠浓度，与假手术组相比较，两药 联用组明显下降(P0．05)。血肌酐浓度，与假手术组相比较，UUO 组及两药联用组肌酐比明显升高(P0．05)，血尿素氮浓度，各实验 组两两比较，无统计学差异(PO．05)。 结论： 1单侧输尿管结扎(UUO)可以快速建立肾间质纤维化动物模型。 2 TGF—B l、FN和Col I的表达增多与肾间质纤维化发生密切 相关。 3螺内酯通过下调肾脏TGF．B l的高表达，减少FN和Col I在 间质的沉积，阻止肾间质纤维化进展。其抗纤维化作用与ACEI相似。 4醛固酮受体拮抗剂与ACEI联用对减轻肾间质纤维化具有协 同作用。 5两药联用的同时增加了血钾增高、肾功能减退的危险性，联 用时须监测肾功能、血电解质。 关键词螺内酯，依那普利，输尿管梗阻，转化生长因子一B。， FN，Col I Ⅱ 硕士研究生论文 硕士研究生论文 英文摘要 ABSTRACT Objective：To investigate whether spironolactone． a mineralocorticoid receptor antagonist,alone Or in combination with enalapril，all angiotensin conveaing enzyme inhibitor(ACEI)， ameliorates renal interstitial fibrosis in all experimental model of complete unilateral ureteral obstruction(troo)． Methods：Thirty male SD rats were randomly assigned to five groups and were treated as follows：shanl operation group(sham group)， UUO group，spironolactone treatment group(50mg．k-1．d．1)，enalapril treatment group (3 lmg．k-1．d-1) and their combination group． Immunohistochemistry were performed to examine the expression of transforming growth factor beta I(TGF-13 1)and fibronectin(nO at the 14”day in the kidney．The expression areas of TGF-B 1 and FN were quantitatively analyzed by image analysis．HE and Masson Trichrome staining were performed to investigate renal pathological changes and the degree of interstitial fibrosis．ne fibrotic areas were also quantitatively analyzed by image analysis．The expression of collagen type 1 were measured by Western