蜜炙远志调控胃肠Cajal间质细胞及其关联机制的研究中药学专业毕业论文.docxVIP

成都中医药大学博士学位论文 成都中医药大学博士学位论文 - - PAGE 10 - ABSTRACT Objective:Expound the biological mechanism of Radix Polygalae reducing the motility disorder in gastrointestinal tract by honey-fried,reveal the scientific connotation of traditional Chinese medicine reducing the stomach and intestines toxic by concoct in molecular level,It has great practical value and the academic significance of using Radix Polygalae reasonably and safely. Methods:Application method of electrophysiology 、 molecular biology immunohistochemistry and so on.To explored the crude Radix P. , its saponins and the honey-stir-baked Radix P. to the motility disorder in gastrointestinal tract,We did the following researches: ①use BL-420 biologic analyzer to observe and record the electrophysiology in stomach and small intestine;②use immunohistochemistry to observe and record ICC in stomach and small intestine;③use RT-PCR and Western Blot to study the expression of c-kit mRNA and c-kit protein、SCF mRNA and SCF protein in stomach and small intestine ;④use HE to observe mucosa damage in stomach and small intestine;⑤use TUNEL to observe the Apoptosis in stomach and small intestine. Result：①Crude Radix P. and its saponins can obviously inhibit slow wave in stomach and small intestine（P＜0.01, P＜0.05）,but Honey-stir-baked Radix P. can not（P＞0.05）;②Crude Radix P. and its saponins can obviously decrease ICC（P＜ 0.01）;Honey-stir-baked Radix P. can not;③Crude Radix P. and its saponins can remarkably decrease the expression of c-kit and SCF in Antral and small intestine（P ＜0.01）;Honey-stir-baked Radix P. can not（P＞0.05）.while Radix P., its saponins and the honey-stir-baked Radix P.can not affect the expression of c-kit and SCF in Gastric pacing District（P＞0.05）;④Crude Radix P. and its saponins can obviously cause the mucosa damage in stomach and small intestine （ P ＜ 0.01 ） ,but Honey-stir-baked Radix P. can not（P＞0.05）;⑤Crude Radix P. and its saponins can obviously increase the Apoptosis in stomach and small