酶生物燃料电池的制备及其性能研究-应用化学专业毕业论文.docxVIP

Abstract II II Abstract Enzyma tic biofuel cell is a device converting chemica l energy directly with the biocatalysts, which has the advantage of abundant fuel resource, mild reaction condition and good biology consistency. In enzymatic biofuel cell aspect, more attention is concentrated on new met hod to immobilized enzyme. The details of preparation met hods of enzymatic electrodes are especia lly described. This paper researched into the preparation and performa nce of single-compartmentalized and membrane elect rode assembly enzymatic biofuel cells . The polymer films have three dimension configurations, which supply much available energy field. There are a lot of active groups in the polymer films. The polymer films have elect rochemica l catalyze character ist ics. Because of its easy preparation and strong attachment to the elect rode surface, the polymer modified elect rode has distinguished itself as one of the most widely used chemica lly modified elect rodes. This work prepared polymer chemica lly modified elect rode, which acted as elect ron med ium of immobilized enzyme elect rode. The ma in research work is as follows: First of all, we prepared poly-(methylene green) chemica lly modified elect rode by cyclic voltammet ric met hod. We studied the PMG/CME of electropolymeriza tion process, character iza tion, elect rochemica l behavior and elect rocatalyzing NADH. The exper iment result is PMG/CME has obvious elect rocatalysis to NADH. We report the assembly of single-compartmentalized enzymatic biofuel cell. The alcohol dehydrogenase (ADH) and NAD+ was immobilized in the PMG/CME by co-electrodepositing met hod as bioa node for the oxidation of alcohol. We used tradition met hod Pt/C catalyzer as cathode, oxygeon as cathodic fuel. Experiment indica ted that the ma ximum open circuit voltage could reach 735 mV. The ma ximum output power density was 62.4 μW/cm2, output potentia l was about 395 mV at an external optima l load of 3 k? and enzyme lif